CBMA

Centro de Biologia Molecular e Ambiental

Centre of Molecular and Environmental Biology

João Azevedo-Silva
João Azevedo-Silva
...
Post-Doc Researcher
Innovation for Life - Functional Genomics and Nanobiotechnology for Sustainable Living
Funtional/Structural membrane transporters characterization
Margarida Casal

Current Research & Key Publications

Project Trans-acids (PTDC/BIA­MIC/5184/2014)

Structural-functional studies of carboxylic acid transporters: Novel tools in production optimization using industrial microbes


Azevedo-Silva J, Queirós O, Ribeiro A, Baltazar F, Young KH, Pedersen PL, Preto A, Casal M. The cytotoxicity of 3-Bromopyruvate in breast cancer cells depends on extracellular pH. Biochem J. 2015 Apr 15;467(2):247-58

Baltazar F, Pnheiro C, Morais-Santos F, Azevedo-Silva J, Queirós O, Preto A, Casal M. Monocarboxylate transporters as targets and mediators in cancer therapy response. Histol Histopathol. 2014 (Epub ahead of print)

Queirós O, Preto A, Pacheco A, Pinheiro C, Azevedo-Silva J, Moreira R, Pedro M, Ko YH, Pedersen PL, Baltazar F, Casal M. Butyrate activates the monocarboxylate transporter MCT4 expression in breast cancer cells and enhances the antitumor activity of 3-bromopyruvate. J Bioenerg Biomembr. 2012 Feb;44(1):141-53.

Pinheiro C, Longatto-Filho A, Azevedo-Silva J, Casal M, Schmitt FC, Baltazar F. Role of monocarboxylate transporters in human cancers: state of the art. J Bioenerg Biomembr. 2012 Feb; 44(1):127-39. 


 

Research Projects

 

 

'The role of Monocarboxylate transporters in 3-bromopyruvate effect in cancer cells'

 

PhD Project, (PhD Grant SFRH/BD/76038/2011 funded by Fundação para a Ciência e Tecnologia beginning in January 2012) supervised by Professor Doctor Margarida Casal and Professor Doctor Ana Preto (co-supervisor).


Research grantee in the scope of the european project ECOPLAST–FP7-NMP-2009-SME-3 under the supervision of Professor Doctor Margarida Casal and Doctor Tony Collins (Biology Department, University of Minho) between October 2010 and December 2011.

Publications

Oliveira CSF, Azevedo-Silva J, Casal M, Côrte-Real M, Baltazar F, Preto A. Characterization of acetate transport in colorectal cancer cells and potential therapeutic implications. Oncotarget 2016 Sep; doi: 10.18632/oncotarget.12156

Azevedo-Silva J, Queirós O, Baltazar F, U?aszewski S, Goffeau A, Ko Y H, Pedersen P L, Preto A, Casal M. The Anticancer Agent 3-Bromopyruvate: A Simple But Powerful Molecule Taken From The Lab To The Bedside. J Bioenerg Biomembr 2016 Aug;48(4):349-62

Azevedo-Silva J, Queirós O, Ribeiro A, Baltazar F, Young KH, Pedersen PL, Preto A, Casal M. The cytotoxicity of 3-Bromopyruvate in breast cancer cells depends on extracellular pH. Biochem J. 2015 Apr 15;467(2):247-58

Baltazar F, Pinheiro C, Morais-Santos F, Azevedo-Silva J, Queirós O, Preto A, Casal M. Monocarboxylate transporters as targets and mediators in cancer therapy response. Histol Histopathol. 2014 (Epub ahead of print)

Collins T*, Azevedo-Silva J*, Costa A*, Branca F, Machado R, Casal M. Batch production of a silk-elastin-like protein in E. coli BL21(DE3): key parameters for optimisation. Microb Cell Fact. 2013 Feb; 12(1):21. (* - equal contributors).

Machado R, Azevedo-Silva J, Correia C, Collins T, Arias FJ, Rodríguez-Cabello JC, Casal M . High level expression and facile purification of recombinant silk-elastin-like polymers in auto induction shake flask cultures. AMB Express. 2013 Feb; 3(1):11.

Queirós O, Preto A, Pacheco A, Pinheiro C, Azevedo-Silva J, Moreira R, Pedro M, Ko YH, Pedersen PL, Baltazar F, Casal M. Butyrate activates the monocarboxylate transporter MCT4 expression in breast cancer cells and enhances the antitumor activity of 3-bromopyruvate. J Bioenerg Biomembr. 2012 Feb;44(1):141-53.

Pinheiro C, Longatto-Filho A, Azevedo-Silva J, Casal M, Schmitt FC, Baltazar F. Role of monocarboxylate transporters in human cancers: state of the art. J Bioenerg Biomembr. 2012 Feb; 44(1):127-39. 

Azevedo-Silva J, Queirós O, Ribeiro A, Baltazar F, Young KH, Pedersen PL, Preto A, Casal M. The cytotoxicity of 3-Bromopyruvate in breast cancer cells depends on extracellular pH. Eur Biophys J 2015, 44 (Suppl 1): S43-S248

Azevedo-Silva J, Queirós O, Ko YH, Pedersen P, Preto A and Casal M. Characterization of 3-bromopyruvate uptake in breast cancer cells. Front. Pharmacol. 2014 Conference Abstract: 4th Annual Meeting of the International Society of Proton Dynamics in Cancer. 

Pacheco A, Queirós O, Preto A, Pinheiro C, Azevedo-Silva J, Moreira R, Pedro M, Baltazar F, Casal M. 3-bromopyruvate cytotoxic effect in breast cancer cells is dependent of monocarboxylate transporters (MCT) expression and is enhanced by butyrate. Exp Clin Endocrinol Diabetes 2012; 120 - A22.

 Collins T, Barroca M, Branca F, Azevedo-Silva J, da Costa A, Machado R, Casal M, “Optimising a cell factory system for the bioproduction of silk-elastin-like polymers”. (2013) 1st Internacional Conference on Natural Fibres (ICNF2013): Sustainable Materials for Advanced Applications, page 127. Raul Fangueiro (Editor), Guimarães, Portugal. ISBN: 978-989-20-3872-8.

Machado R, da Costa A, Sencadas V, Azevedo-Silva J, Collins T, Arias F J, Lanceros-Mendez S, Rodríguez-Cabello J C, Casal M, “Synthesis and processing of recombinant silk and elastin  copolymers”. (2013) 1st Internacional Conference on Natural Fibres (ICNF2013): Sustainable Materials for Advanced Applications, page 153. Raul Fangueiro (Editor), Guimarães, Portugal. ISBN: 978-989-20-3872-8

Campos A R, Oliveira B, Machado R, Collins T, Azevedo-Silva J, da Costa A, Immonen K, Wikström L, Casal M, “Novel nanocellulose reinforced recombinant protein based polymer composites”. (2013) 1st Internacional Conference on Natural Fibres (ICNF2013): Sustainable Materials for Advanced Applications, page 333. Raul Fangueiro (Editor), Guimarães, Portugal. ISBN: 978-989-20-3872-8

 


Cazzanelli G, Moreira T, Ferro S, Azevedo-Silva J, Nogueira E, Preto A. Colorectal cancer therapeutic approaches: from classical drugs to new nanoparticles. Frontiers in Anti-Cancer Drug Discovery, 2016, vol. 7, 3-82

 

 

Oral Communications

Transport of short chain fatty acids and 3-bromopyruvate in cancer cells: implications for cancer therapy. Azevedo-Silva J, Oliveira CS, Queirós O, Ribas D, Côrte-Real M, Baltazar F, Goffeau A, Ko YH, Pedersen PL, Preto A, Casal M. XIX Congresso Nacional de Bioquímica, Guimarães (Portugal), December 2016.

Transport of monocarboxylic acids in cancer cells: implications of short chain fatty acids and 3-bromopyruvate for cancer therapy. Azevedo-Silva J, Oliveira CS, Queirós O, Ribas D, Côrte-Real M, Baltazar F, Goffeau A, Ko YH, Pedersen PL, Preto A, Casal M. Small meeting on yeast transport and energetics, Chania (Greece), August 2016

Uncovering the molecular structure of the AceTr transporter family. Ribas D, Azevedo-Silva J, Athayde D, Archer M, Casal M. Small meeting on yeast transport and energetics, Chania (Greece), August 2016

The extracellular pH influences the cytotoxicity and transport of 3-Bromopyruvate by monocarboxylate transporters in breast cancer cells. Azevedo-Silva J, Queirós O, Ribeiro A, Baltazar F, Ko YH, Pedersen P, Preto A, Casal M. XL Jornadas Portuguesas de Genética, Coimbra (Portugal), Junho 2016

The anticancer selectivity of 3-Bromopyruvate is dependent on MCTs activity and extracellular pH: a kinetic approach. Azevedo-Silva J. DBio Seminars, Department of Biology - University of Minho, 22 January 2014 (invited communication)

Characterization of 3-bromopyruvate uptake in breast cancer cells. Azevedo-Silva J, Queirós O, Ko YH, Pedersen PL, Preto A,Casal M.The 4th Annual Meeting of the International Society of Proton Dynamics in Cancer, Munich (Germany), October 2013.

Synthesis and processing of recombinant silk and elastin copolymers. Machado R, da Costa A, Sencadas V, Azevedo-Silva J, Collins T, Arias FJ, Lanceros-Mendez S, Rodríguez-Cabello JC, Casal M. 1st Internacional Conference on Natural Fibres (ICNF2013), Guimarães (Portugal), June 2013. 

Optimising a cell factory system for the bioproduction of silk-elastin-like polymers. Collins T, Barroca M, Branca F, Azevedo-Silva J, da Costa A, Machado R, Casal M. 1st Internacional Conference on Natural Fibres (ICNF2013), Guimarães (Portugal), June 2013.

Transport of lactic acid across plasma membrane: implications in health and biotechnology.  Casal M, Pacheco A, Sá-Pessoa J, Azevedo-Silva J, Preto A, Pinheiro P, Ko YH, Pedersen PL, U?aszewski W, Goffeau A, Baltazar F, Paiva S, Queirós O. SMYTE 30, Salamanca (Spain), July 2012.

Protein-engineered hybrid biomaterials: molecular genetic strategies for recombinant proteins expression in Escherichia coli. Azevedo-Silva J. DBio Seminars, Department of Biology - University of Minho, 3 November 2010 (invited communication).


Poster Communications

 

Granja S, Azevedo-Silva, J, Queirós O, Preto A, Baltazar F, Casal M. “Targeting glycolysis in acute myeloid leukemia: therapeutic applications”. XIX Congresso Nacional de Bioquímica, Guimarães (Portugal), December 2016

Azevedo-Silva J, Queirós O, Ribeiro A, Baltazar F, Ko YH, Pedersen P, Preto A, Casal M. “The extracellular pH influences the cytotoxicity and transport of 3-Bromopyruvate in breast cancer cells”. 2nd ASPIC International Congress, Porto (Portugal), April 2016

Azevedo-Silva J, Queirós O, Ribeiro A, Baltazar F, Ko YH, Pedersen P, Preto A, Casal M. “The cytotoxicity of 3-Bromopyruvate in breast cancer cells depends on extracellular pH”. 10th European Biophysics Congress, Dresden (Germany), July 2015

Azevedo-Silva J, Baltazar F, Ko YH, Petersen PL, Preto A, Casal M. “Silencing of Monocarboxylate transporters as a tool to study the role of 3-bromopyruvate in breast cancer”. XXXIX Jornadas Portuguesas de Genética, Braga (Portugal), May 2015

Azevedo-Silva J, Queirós O, Ribeiro A, Baltazar F, Ko YH, Pedersen P, Preto A, Casal M. “The cytotoxicity of 3-Bromopyruvate in breast cancer cells depends on extracellular pH”. 2as Jornadas do programa doutoral em Biologia Molecular e Ambiental, Braga (Portugal), November 2014

Azevedo-Silva J, Queirós O, Ko YH, Pedersen PL, Preto A, Casal M. “Characterization of 3-bromopyruvate uptake in breast cancer cells”. 1as Jornadas do programa doutoral em Biologia Molecular e Ambiental, Braga (Portugal), November 2013

Campos AR, Oliveira B, Machado R, Collins T, Azevedo-Silva J, da Costa A, Immonen K, Wikström L, Casal M. “Novel nanocellulose reinforced recombinante protein based polymer composites”. 1st Internacional Conference on Natural Fibres (ICNF2013), Guimarães (Portugal), June 2013.

Collins T, Azevedo-Silva J, da Costa A, Branca F, Machado R, Casal M. “Key stress factors and parameters for batch production optimisation of silk-elastin-like proteins in E. coli”. 7th Conference on Recombinant Protein Production (RPP7), Laupheim (Germany), March 2013. 

Pacheco A, Queirós O, Preto A, Pinheiro C, Azevedo-Silva J, Moreira R, Pedro M, Ko YH, Pedersen PL, Baltazar F, Casal M. “Butyrate activates the monocarboxylate transporter MCT4 expression in breast cancer cells and enhances the antitumor activity of 3-bromopyruvate”. XXI Porto Cancer Meeting, Porto, April 2012.

Collins T., Azevedo-Silva J., da Costa A, Machado R., Casal M., “Key stress factors and parameters for batch production optimisation of silk-elastin-like proteins in E. coli”, Microbiotec’11, Braga, December 2011.

Azevedo-Silva J., da Costa A., Machado R., Rodriguez-Cabello J. C., Casal M., “Designing a cost-effective protein purification protocol using Elastin Like Polymer (VPAVG)200 fusion tag”, XXXVI Jornadas de Genética, Coimbra, May 2011.

Azevedo-Silva J., da Costa A., Machado R., Rodríguez-Cabello J.C. and Casal M., “Use of Elastin Like Polymer (VPAVG)200 fusion tags for recombinant protein production and purification”, XVII Congresso Nacional de Bioquímica, Porto, December 2010.

 Azevedo-Silva J., Machado R., Reis R.L.,Rodríguez-Cabello J.C.and Casal M. (2010) “Creating new functional biomaterials: construction and production of Bone Morphogenetic Protein 2-ELP hybrid protein”, XXXV Jornadas Portuguesas de Genética, Braga, June 2010.


 

Post-Docs & Students

 

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