CBMA

Centro de Biologia Molecular e Ambiental

Centre of Molecular and Environmental Biology

Diana Barros
Diana Barros
...
PhD Student
Sustaining Life - Biodiversity and Functional Ecology
Proteomic and physiological responses to metal nanoparticles in aquatic microbes
Fernanda Cássio, Cláudia Pascoal e Pedro M Santos
Centre of Molecular and Environmental Biology (CBMA)
Department of Biology
University of Minho
Campus de Gualtar
4710-057 Braga
Portugal

Current Research & Key Publications

Transcriptomic, proteomic and physiological responses to silver nanoparticles in aquatic microbes

 

 

 

Barros D, Oliveira P, Pascoal C and Cássio F. 2016. Ethanol and phenanthrene increase the biomass of fungal assemblages and decrease plant litter decomposition in streams. Sci Total Environ. 565: 489-495. DOI: 10.1016/j.scitotenv.2016.04.190. (2016 IF: 4.900; Environmental Sciences-Q1)


Research Projects

PhD Student (FCT fellow)

The goal of this project is to assess transcriptomic,  proteomic and physiological responses to Ag NP exposure in aquatic microbes that play key ecological roles in freshwaters. We selected aquatic fungi and bacteria aiming to identify potential biomarkers of exposure and to elucidate the action mechanisms of Ag NPs. The specific objectives of this project are: i) to evaluate the toxicity of Ag NPs to microbes by establishing dose response curves to Ag NP exposure and using microbial growth as endpoint; ii) to assess if exposure to Ag NPs induce oxidative stress in aquatic microbes; iii) to examine changes in the activity of antioxidant enzymes due to Ag NP exposure; iv) to address if Ag NP exposure can cause genotoxicity, as nuclear morphological alterations and DNA strand breaks; and v) to characterize key protein signatures by examining changes in protein expression promoted by exposure to Ag NPs.

NANOECOTOX - Impacts of metal nanoparticles to aquatic ecosystems: from community responses to cellular targets (PTDC/AAC-AMB/121650/2010)

“Unraveling the specific involvement of the small Rho-like GTPase Cdc42 in the highly polymorphic nature of Paracoccidioides brasiliensis yeast cells” (PTDC/BIA-MIC/108309/2008)

Biotest - Biotestes marinhos para a detecção em tempo real da toxicidade do petróleo (POCI/MAR/56964/2004)

Publications

 


 

 

Menino JF, Osório NS, Sturme MH, Barros D, Gomes-Alves AG, Almeida AJ, Ludovico P, Costa P, Goldman GH, Rodrigues F. 2012. Morphological heterogeneity of Paracoccidioides brasiliensis: relevance of the Rho-like GTPase PbCDC42. Med Mycol. 50(7):768-74.

Oral communications by invitation

Barros D, Pradhan A, Mendes V, Manadas B, Santos PM, Pascoal C and Cássio F. Proteomic and antioxidant enzymatic responses to nanoparticulate and ionic silver in Pseudomonas sp. M1. Advanced Course in Genomics, Metagenomics and Bioinformatics at the Centre of Molecular and Environmental Biology (CBMA), University of Minho, Braga, Portugal, July 2013.

 Oral communications

Barros D, Pradhan A, Santos PM, Pascoal C and Cássio F. Proteomic responses to nanoparticulate and ionic silver in freshwater microbes with different background. SETAC Europe 28th Annual Meeting, Rome, Italy, May 2018.

Barros D, Pradhan A, Pascoal C and Cássio F. Proteomic responses to nanoparticulate and ionic silver in fungi from metal-polluted and non-polluted streams. 1st International Conference on Microbial Ecotoxicology, “EcotoxicoMic”, Lyon, France, November 2017.

Menino J, Barros D, Gomes-Alves AG, Hernández O, Almeida AJ and Rodrigues F. Morphological heterogeneityof Paracoccidioides brasiliensis: characterization and relevance of the Rho-like GTPase Pbcdc42. 26th Fungal Genetics Conference, Asilomar, United States of America, March 2013.

 

Poster communications

Pradhan A, Barros D, Pascoal C and Cássio F. Proteomic approach to assess the impacts of engineered nanoparticles on freshwater fungi. XIX Congress of the Iberian Society of Limnology, Coimbra, Portugal, June 2018.

Barros D, Pradhan A, Pascoal C and Cássio F. Proteomic responses to nanoparticulate and ionic silver in aquatic fungi. XIX National Congress of Biochemistry, Guimarães, Portugal, December 2016.

Barros D, Pradhan A, Santos PM, Pascoal C and Cássio F. Proteomic analysis of aquatic microbial responses to nanoparticulate and ionic silver. XXXIII Congress of the International Society of Limnology, Turin, Italy, July/August 2016.

Barros D, Pradhan A, Santos PM, Pascoal C and Cássio F. Responses of antioxidant enzymes to nanoparticulate and ionic silver in aquatic microbes. SETAC Europe 25th Annual Meeting, Barcelona, Spain, May 2015.

Barros D, Oliveira P, Pascoal C and Cássio F. Effects of ethanol and phenanthrene on litter decomposition by aquatic fungal assemblages. 7th Symposium for European Freshwater Sciences, Girona, Spain, June 2011.

Almeida AJ, Barros D, Hernández O, Oliveira P, McEwen J, Ludovico P and Rodrigues F. Paracoccidioides brasiliensis yeast cells do not obey standard rules during cell growth. National Congress of Microbiology, “MICROBIOTEC09”, Vilamoura, Portugal, November 2009.

Post-Docs & Students

 

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