CBMA

Centro de Biologia Molecular e Ambiental

Centre of Molecular and Environmental Biology

André Costa
André Costa
...
Post-Doc Researcher
Innovation for Life - Functional Genomics and Nanobiotechnology for Sustainable Living
Functionalization, design and bioproduction of Protein-Based Polymers
Raul Machado
Departamento de Biologia,
Universidade do Minho - Campus de Gualtar, - 4710-057, Braga - Portugal

Current Research & Key Publications

AREA OF CURRENT SCIENTIFIC ACTIVITY:

- Molecular biology/biotechnology
- Materials Science
- Design and production of functional recombinant protein-based polymers
- Materials with antimicrobial properties

TECHNICAL SKILLS:

- Molecular biology procedures
- Antimicrobial in vitro and ex vivo testing (with two specializations)
- Materials with antimicrobial properties
- Microbiology
- Production of recombinant proteins in Escherichia coli (batch and fed batch)
- Protein purification techniques
- In vitro mammalian cell culture
- Polymeric materials characterization techniques
- Python and HTML5/CSS3 programming
- Web Design

 

- Pereira A.M.*, Machado R.*, da Costa A., Ribeiro A., Bernardo C.T., Collins T., Gomes A.C., Leonor I.B., Kaplan D., Reis R.L., Casal M. “Silk-based polymers functionalized with fibronectin type II for improved cell adhesion” – Acta Biomaterialia, Acta Biomaterialia, Available online 11 November 2016 (* - equal contributors)

- Machado R., da Costa A., Sencadas V., Pereira A.M., Collins T., Rodríguez-Cabello J.C., Lanceros-Méndez S., Casal M. “Exploring the Properties of Genetically Engineered Silk-Elastin-Like Protein Films” Macromol. Biosci. 2015, 15 (12);1698-1709

- da Costa A.*, Machado R.*, Ribeiro A., Thiagarajan V., Neves-Petersen M.T., Rodríguez-Cabello J.C., Gomes A.C., Casal M. “Development of elastin-like recombinamer films with antimicrobial activity” Biomacromolecules. 2015, 16 (2) (* - equal contributors)

- Machado R., da Costa A., Sencadas V., Garcia-Arévalo C., Costa C.M., Padrão J., Gomes A., Lanceros-Méndez S., Rodríguez-Cabello J.C., Casal M. “Electrospun silk-elastin-like fibre mats for tissue engineering applications”, Biomedical Materials. 2013; 8.

- Collins T.*, Azevedo-Silva J.*, da Costa A.*, Branca F., Machado R., Casal M. “Batch production of a silk-elastin-like protein in E. coli BL21(DE3): key parameters for optimisation”. Microb Cell Fact. 2013 Feb; 12(1):21. (* - equal contributors)

Research Projects

 

 

Publications

- Pereira A.M.*, Machado R.*, da Costa A., Ribeiro A., Bernardo C.T., Collins T., Gomes A.C., Leonor I.B., Kaplan D., Reis R.L., Casal M. “Silk-based polymers functionalized with fibronectin type II for improved cell adhesion” – Acta Biomaterialia, Acta Biomaterialia, Available online 11 November 2016 (* - equal contributors)

- Machado R., da Costa A., Sencadas V., Pereira A.M., Collins T., Rodríguez-Cabello J.C., Lanceros-Méndez S., Casal M. “Exploring the Properties of Genetically Engineered Silk-Elastin-Like Protein Films” Macromol. Biosci. 2015, 15 (12);1698-1709.

- da Costa A.*, Machado R.*, Ribeiro A., Thiagarajan V., Neves-Petersen M.T., Rodríguez-Cabello J.C., Gomes A.C., Casal M. “Development of elastin-like recombinamer films with antimicrobial activity” Biomacromolecules. 2015, 16 (2) (* - equal contributors)

- Machado R., da Costa A., Sencadas V., Garcia-Arévalo C., Costa C.M., Padrão J., Gomes A., Lanceros-Méndez S., Rodríguez-Cabello J.C., Casal M. “Electrospun silk-elastin-like fibre mats for tissue engineering applications”, Biomedical Materials. 2013; 8.

- Collins T.*, Azevedo-Silva J.*, da Costa A.*, Branca F., Machado R., Casal M. “Batch production of a silk-elastin-like protein in E. coli BL21(DE3): key parameters for optimisation”. Microb Cell Fact. 2013 Feb; 12(1):21. (* - equal contributors)

 

- da Costa A., Machado R., Ribeiro A., Gomes A., Rodríguez-Cabello J.C., Casal M. “Development of an ex vivo assay for the characterization of a new elastin-like polymer with antimicrobial properties”. 2013. Journal of Tissue Engineering and Regenerative Medicine, 7, Suplement s1, Page 11 (TS11)

- Machado R., da Costa A., Sencadas V., Gomes A., Lanceros-Mendez S., Rodríguez-Cabello J.C., Casal M. “Electrospun recombinant Protein-Based Polymers fibre matrices for skin regeneration”. 2013. Journal of Tissue Engineering and Regenerative Medicine, 7, Suplement s1, Page 12 (TS14)

- Pereira A.M., Bernardo T.C., da Costa A., Collins T., Machado R., Leonor I.B., Casal M., Reis R.L. “Development of new chimeric proteins for tissue engineering”. 2013. Journal of Tissue Engineering and Regenerative Medicine, 7, Suplement s1, Page 11 (TS11).

- Pereira A.M., Bernardo T.C., da Costa A., Collins T., Machado R., Leonor I.B., Casal M., Reis R.L. “Development of new chimeric proteins for tissue engineering”. 2013. Journal of Tissue Engineering and Regenerative Medicine, 7, Suplement s1, Page 46 (POL07).

- Collins T., Barroca M., Branca F., Azevedo-Silva J., da Costa A., Machado R., Casal M. “Optimising a cell factory system for the bioproduction of silk-elastin-like polymers”. 2013. 1st Internacional Conference on Natural Fibres (ICNF2013): Sustainable Materials for Advanced Applications, page 127. Raul Fangueiro (Editor), Guimarães, Portugal. ISBN: 978-989-20-3872-8

- Machado R., da Costa A., Sencadas V., Azevedo-Silva J., Collins T., Arias F.J., Lanceros-Mendez S., Rodríguez-Cabello J.C., Casal M. “Synthesis and processing of recombinant silk and elastin copolymers”. 2013. 1st Internacional Conference on Natural Fibres (ICNF2013): Sustainable Materials for Advanced Applications, page 153. Raul Fangueiro (Editor), Guimarães, Portugal. ISBN: 978-989-20-3872-8

- Campos A.R., Oliveira B., Machado R., Collins T., Azevedo-Silva J., da Costa A., Immonen K., Wikström L., Casal M. “Novel nanocellulose reinforced recombinant protein based polymer composites”. 2013. 1st Internacional Conference on Natural Fibres (ICNF2013): Sustainable Materials for Advanced Applications, page 333. Raul Fangueiro (Editor), Guimarães, Portugal. ISBN: 978-989-20-3872-8

 

 

 

 PODIUM COMMUNICATIONS BY INVITATION:

da Costa A. “The importance of polymers in the antimicrobial world” Advanced Course on "Biopolymers based on renewable resources: from synthesis to applications (3rd Edition)" 11-22 April 2016, Universidade do Minho, Braga, Portugal

- da Costa A. “Functionalization of Protein-based Polymers for advanced biomaterials” DBio Seminars. 9th December 2015, Universidade do Minho, Braga, Portugal

da Costa A. and Machado R. “Methodologies for the assessment of biopolymer's antimicrobial activity” Advanced Course on "Biopolymers based on renewable resources: from synthesis to applications (2nd Edition)" 7-17 April 2015, Universidade do Minho, Braga, Portugal

da Costa A. “Development of a recombinant protein-based polymer exhibiting antimicrobial properties” DBio Seminars. 15th January 2014, Universidade do Minho, Braga, Portugal

- da Costa A. “Functionalization of Elastin-Like Polymers (ELPs) as antimicrobial materials” DBio Seminars. 31st October 2012, Universidade do Minho, Braga, Portugal

 

 

PODIUM COMMUNICATIONS:

 

da Costa A., Machado R., Rodríguez-Cabello J.C., Gomes A.C., Casal M. “Creation of antimicrobial biopolymers by the use of recombinant DNA technology” XL Portuguese Genetics Conference, June 3 2016, Coimbra, Portugal.

- Pereira A.M., Machado R., Bernardo T., da Costa A., Ribeiro A., Collins T., Gomes A.C., Leonor I., Kaplan D.L., Reis R.L., Casal M. “Genetically engineered silk-based composite biomaterials functionalized with fibronectin type-II that promote cell adhesion”. XL Portuguese Genetics Conference, June 3 2016, Coimbra, Portugal.

- Pereira A.M., Machado R., Bernardo T., da Costa A., Ribeiro A., Collins T., Gomes A.C., Leonor I., Kaplan D.L., Reis R.L., Casal M. “Advanced silk-based genetic polymers with improved cell adhesion properties”. MicroBiotec’15, December 10 – 12, 2015, Évora, Portugal.

da Costa A., Machado R., Ribeiro A., Collins T., Thiagarajan V., Neves-Petersen M. T., Rodríguez-Cabello J.C., Gomes A.C., Casal M. “Synthetic Protein Biotechnology approaches for the creation of antimicrobial biopolymers” XXXIX Portuguese Genetics Conference, May 25-27 2015, Braga, Portugal.

da Costa A., Machado R., Sencadas V., Lanceros-Méndez S., Gomes A.C., Rodriguez-Cabello J.C., Casal M. “Electrospun fibres of an elastin-like recombinamer functionalized with an antimicrobial domain” ICNF2015, April 27-29 2015, S. Miguel, Açores, Portugal

Machado R., Silva A.M., da Costa A., Gomes A.C., Rodríguez-Cabello J.C., Lanceros-Méndez S., Sencadas V., Casal M. “Antimicrobial electrospun membranes of SELP/Ag composites” ICNF2015, April 27-29 2015, S. Miguel, Açores, Portugal

Campos A.R., Oliveira B., Machado R., Collins T., Azevedo-Silva J., da Costa A., Immonen K., Wikström L., Casal M. “Nanocellulose composites using a recombinant protein based polymer as a matrix” ICNF2015, April 27-29 2015, S. Miguel, Açores, Portugal

da Costa A., Machado R., Ribeiro A., Collins T., Viruthachalam T., Neves-Petersen M.T., Rodríguez-Cabello J.C., Gomes A.C., Casal M. “A new highly antimicrobial bio-inspired protein-based polymer designed for medical devices” ICAR2014, October 1-3 2014, Madrid, Spain

da Costa A., Machado R., Ribeiro A., Gomes A., Rodríguez-Cabello J.C., Casal M. “Antibacterial and antifungal activity of a new solvent-cast film based on a functionalized elastin-like polymer” MICROBIOTEC ’13, December 6-8 2013, Aveiro, Portugal

Machado R., da Costa A., Sencadas V., Gomes A., Lanceros-Mendez S., Rodríguez-Cabello J.C., Casal M. “Electrospun recombinant Protein-Based Polymers fibre matrices for skin regeneration” Term Stem 2013, October 10-12 2013, Porto, Portugal

da Costa A., Machado R., Ribeiro A., Gomes A., Rodríguez-Cabello J.C., Casal M. “A synthetic biology approach for the development of recombinant protein-based polymers exhibiting antimicrobial properties” European Symposium on Biopolymers (ESBP 2013), October 7- 9 2013, Lisboa, Portugal.

Collins T., Barroca M., Branca F., Azevedo-Silva J., da Costa A., Machado R., Casal M. “Optimising a cell factory system for the bioproduction of silk-elastin-like polymers” 1st International Conference on Natural Fibres (ICNF2013), June 9-11 2013, Guimarães, Portugal.

Machado R., da Costa A., Sencadas V., Azevedo-Silva J., Collins T., Arias F.J., Lanceros-Mendez S., Rodríguez-Cabello J.C., Casal M. “Synthesis and processing of recombinant silk and elastin copolymers” 1st International Conference on Natural Fibres (ICNF2013), June 9-11 2013, Guimarães, Portugal.

- da Costa A., Machado R., Casal M.  “Solvent-cast films of an elastin-like polymer fused to an antimicrobial peptide, ABP-CM4, exhibits high antibacterial activity against P. aeruginosa”, Microbiotec’11, December 2011, Braga, Portugal

POSTER COMMUNICATIONS:

- Gomes, D., da Costa A., Pereira A.M., Casal M., Machado R., “Formulation of biocomposites based on recombinant proteins and plant essential oils for the development of active wound dressings”, XL Portuguese Genetics Conference, June 3 2016, Coimbra, Portugal

Machado R., Silva A.M., da Costa A., Gomes A.C., Sencadas V., Casal M., “Antimicrobial activity of genetically engineered silk-elastin-like composite materials”, XL Portuguese Genetics Conference, June 3 2016, Coimbra, Portugal.

da Costa A., Machado R., Sencadas V., Lanceros-Méndez S., Gomes A.C., Rodriguez-Cabello J.C., Casal M. “Electrospun fibres of an elastin-like recombinamer functionalized with an antimicrobial domain”, Microbiotec’15, December 10 – 12, 2015, Évora, Portugal

Machado R., Silva A.M., da Costa A., Gomes A.C., Rodríguez-Cabello J.C., Lanceros-Méndez S., Sencadas V., Casal M. “Fabrication of antimicrobial composite materials comprising a genetically engineered copolymer”. MicroBiotec’15, December 10 – 12, 2015, Évora, Portugal

Pereira A.M., Machado R., Bernardo T.C., da Costa A., Ribeiro A., Collins T., Gomes A.C., Leonor I., Kaplan D., Reis R.L., Casal M. “Silk-based polymers functionalized with fibronectin type-II for improved cell adhesion” III symposium of the PDBMA, November 26, 2015, Braga, Portugal

Pereira A.M., Viana J.F.C., da Costa A., Machado R., Ferreira A.C.R., Casal M., Franco O.L., Dias S.C. “Heterologous expression of antimicrobial peptide synoeca-mp fused to recombinant protein-based polymer as platform for biomaterials” 23rd International Congress of the International Union for Biochemistry and Molecular Biology (IUBMB) and 44th Annual Meeting of the Brazilian Society for Biochemistry and Molecular Biology, 24 – 28 August 2015, Foz do Iguaçu, PR, Brazil.

Machado R., Silva A.M., da Costa A., Gomes A.C., Rodríguez-Cabello J.C., Lanceros-Mendez S., Sencadas V., Casal M. “Antibacterial activity of genetically engineered silk-elastin-like films and fibres containing silver nanoparticles” XXXIX Jornadas Portuguesas de Genética, May 25-27, 2015, Braga, Portugal

Pereira A.M., Machado R., Bernardo T., da Costa A., Ribeiro A., Collins T., Gomes A.C., Leonor I., Kaplan D.L., Reis R.L., Casal M. “Silk-based polymers functionalized with fibronectin type-II for improved cell adhesion”XXXIX Jornadas Portuguesas de Genética, May 25-27, 2015, Braga, Portugal

Pereira A.M., Machado R., da Costa A., Ribeiro A., Collins T., Gomes A.C., Leonor I.B., Reis R.L., Casal M. “Functionalized silk-based polymers as advanced biomaterials” II Symposium of the PDBMA, November 27, 2014, Braga, Portugal

Silva A.M., da Costa A., Chitas R., Collins T., Gomes A., Rodríguez-Cabello J.C., Lanceros-Méndez S., Sencadas V., Casal M, Machado R. “Fabrication of SELP/Ag nanocomposite materials with antimicrobial properties by electrospinning and solvent casting" International Conference on Antimicrobial Research (ICAR 2014), October 1-3, 2014, Madrid, Spain

Machado R., da Costa A., Sencadas V., Gomes A., Lanceros-Mendez S., Rodríguez-Cabello J.C., Casal M. “Electrospun silk-elastin-like fiber mats for tissue engineering applications” Microbiotec’13, December 6-8, 2013, Aveiro, Portugal

Machado R., da Costa A., Sencadas V., Gomes A., Lanceros-Mendez S., Rodríguez-Cabello J.C., Casal“Electrospun silk-elastin-like fiber mats with potential application for tissue engineering” Electrospinning, Principles, Possibilities and Practice 2013, December 5-6, 2013, Institute of Physics, London, UK

da Costa A., Machado R., Ribeiro A., Gomes, A., Rodriguez-Cabello J.C., Casal M. “Antibacterial and antifungal activity of a new solvent-cast film based on a functionalized elastin-like polymer” I Symposium of the PDBMA, November 28, 2013, Braga, Portugal

Pereira A.M., Bernardo T.C., da Costa A., Collins T., Machado R., Leonor I.B., Casal M., Reis R.L. “Development of New Chimeric Proteins for Tissue Engineering” I Symposium of the PDBMA, November 28, 2013, Braga, Portugal

da Costa A., Machado R., Ribeiro A., Gomes A., Rodríguez-Cabello J.C., Casal M. “Development of functionalized recombinant protein-based polymers exhibiting antimicrobial properties” Materials Today Virtual Conference: Biomaterials, November 19-21, 2013, online conference (http://www.materialstoday.com/virtualconference/materials-today-virtual-conference-biomaterials-2013)

Machado R., da Costa A., Sencadas V., Gomes A., Lanceros-Mendez S., Rodríguez-Cabello J.C., Casal M. “Fabrication of recombinant silk-elastin-like fiber mats for tissue engineering applications” Materials Today Virtual Conference: Biomaterials, November 19-21, 2013, online conference (http://www.materialstoday.com/virtualconference/materials-today-virtual-conference-biomaterials-2013)

da Costa A., Machado R., Ribeiro A., Gomes A., Rodríguez-Cabello J.C., Casal M. “Development of an ex vivo assay for the characterization of a new elastin-like polymer with antimicrobial properties” Term Stem 2013, October 10-12, 2013, Porto, Portugal

Pereira A.M., Bernardo T.C., da Costa A., Collins T., Machado R., Leonor I.B., Casal M., Reis R.L. “Development of new chimeric proteins for tissue engineering” Term Stem 2013, October 10-12, 2013, Porto, Portugal

Pereira A.M., Bernardo T.C., da Costa A., Collins T., Machado R., Leonor I.B., Casa M. l, Reis R.L. “Development of new chimeric proteins for tissue engineering” 1st Polaris Workshop, October 7-9, 2013, Porto, Portugal.

Machado R., da Costa A., Sencadas V., Gomes A., Lanceros-Mendez S., Rodríguez-Cabello J.C., Casal M. “Electrospun silk-elastin-like fibre matrices for tissue engineering applications” European Symposium on Biopolymers (ESBP 2013), October 7-9 2013, Lisboa, Portugal.

Pereira A.M., Bernardo T.C., da Costa A., Collins T., Machado R., Leonor I.B., Casal M., Reis R.L. “Development of new chimeric proteins for tissue engineering” 3rd ICVS/3B's Meeting, June 28, 2013, Braga, Portugal.

Collins T., Azevedo-Silva J., da Costa A., Machado R., Casal M. “Key stress factors and parameters for bath production optimisation of silk-elastin-like proteins in E. coli” Microbiotec’11, Braga, December 2011

da Costa A., Azevedo-Silva J., Machado R., Rodriguez-Cabello J. C., Casal M.”Designing a cost-effective protein purification protocol using Elastin Like Polymer (VPAVG)200 fusion tag” XXXVI Jornadas Portuguesas de Genética, Coimbra, May 2011

Azevedo-Silva J., da Costa A., Machado R., Rodríguez-Cabello J.C. and Casal M. “Use of Elastin Like Polymer (VPAVG)200 fusion tags for recombinant protein production and purification” XVII Congresso Nacional de Bioquímica, Porto, December 2010

- da Costa A., Machado R., Matamá T., Rodriguez-Cabello J.C., Cavaco-Paulo A., Casal M. “Production and purification of recombinant antimicrobial peptides by exploiting the termoresponsiveness of the elastin-like polymer based on the VPAVG sequence”, XXXV Jornadas Portuguesas de Genética, Braga, June 2010

 

Post-Docs & Students

 

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