CBMA

Centro de Biologia Molecular e Ambiental

Centre of Molecular and Environmental Biology

Ana Preto
Ana Preto
...
253601524
Assistant Professor/Researcher
Basics of Life - Cellular Responses to Environmental Stress
Universidade do Minho - Campus de Gualtar, Departamento de Biologia - 4710-057, Braga - P

Current Research & Key Publications

Ana Preto is an Assistant Professor at the Department of Biology and senior researcher at CBMA in the University of Minho, and Consultant Researcher at IPATIMUP/i3S. She graduated in Pharmaceutical Sciences from the Faculty of Pharmacy in 1998 and completed her PhD in Human Biology by the Faculty of Medicine in 2004, both at the University of Porto. Ana Preto was a PhD student of the PhD Program GABBA and performed the PhD in IPATIMUP in collaboration with the Department of Pathology, University of Wales College of Medicine, Cardiff, Wales, UK (2000/2004). From 2004 to 2007 had a Post-Doc position at IPATIMUP.

Ana Preto has been focusing in understanding the role of KRAS mutations signalling pathways in autophagy regulation in colorectal cancer (CRC) survival, towards identification of new molecular targets to metastatic CRC. Ana Preto has also been interested in unravelling in CRC, the mechanism of action of short chain fatty acids namely acetate produced by propionibacteria from diet. Ana Preto goal is the identification of new molecular targets for CRC, which could inspire other groups, with whom she collaborates, to the development of new drugs or nanoparticles for specific target of CRC cells.

Ana Preto is Reviewer at 15 peer-reviewed journals and was an Invited evaluator for research PhD grants at the "Fonds National de la Recherche (FNR)”, Luxembourg and invited evaluator  for research projects contest from the “Gabinete de Apoio à Investigação (GAI)”,  from the faculty of Medicine from the University of Coimbra. She is Topic Associated Editor at Frontiers in Nutrition. She has been involved in the organising committee of both National and International meetings. Granted 4 prizes and 3 grants; supervised 37 post-graduate students including 24 Master students (4 ongoing) and 6 PhD students (2 ongoing). Participated as team researcher in several research projects: 12 National, 2 European (1 FP7 and 1 Marie Curie) and 1 USA (financed by KoDiscoveryâ company). Co-authored 2 Patents.

Ana Preto has 63 Scientific Publications (49 Papers in scientific journals with peer review indexed in ISI; 14 Abstracts published in proceedings of scientific meetings), 1 book chapter, with more than 1980 citations (Scopus) and 83 Scientific Communications (24 Oral communications and 59 Posters). She is the first author in 7 manuscripts and senior author in 5, with an h-index (Scopus) of 20. Her results were published in top cancer-related journals, including Oncogene, BMC Cancer, Journal of Pathology, Cell Death and Disease, Oncotarget. Ana Preto also participates in several projects not directly related with her own research, with data published in Q1 journals, such as Nature Communications, Br J Cancer, Biochemistry Journal, Cancer Letters, Nanomedicine, Biomacromolecules, Faraday Discussions, Journal of Biomedical Nanotechnology, Modern Pathology reflecting her multidisciplinary scientific expertise.


Ferro S, Azevedo-Silva J, Casal M, Côrte-Real M, Baltazar F, Preto A. “Characterization of acetate transport in colorectal cancer cells and potential therapeutic implications”, Oncotarget. 2016 Sep 21. doi: 10.18632/oncotarget.12156. Impact Factor: 6.359

Alves S, Castro L, Fernandes MS, Francisco R, Castro P, Priault M, Chaves SR, Moyer MP, Oliveira C, Seruca R, Côrte-Real M, Sousa MJ, Preto A. “Colorectal cancer-related mutant KRAS alleles function as positive regulators of autophagy”. Oncotarget. 2015 Oct 13;6(31):30787-802. doi: 10.18632/oncotarget.5021. Impact Factor: 6.359

Oliveira CSF, Pereira H, Alves S, Castro L, Baltazar F, Chaves SR, Côrte-Real M, Preto A."Cathepsin D protects colorectal cancer cells from acetate-induced apoptosis through autophagy-independent degradation of damaged mitochondria". Cell Death Dis. 2015 Jun 18;6:e1788. doi: 10.1038/cddis.2015.157. Impact Factor: 5.378

Marques C, Oliveira CSF, Alves S, Chaves SR, Coutinho OP, Côrte-Real M, Preto A. “Acetate-induced apoptosis in colorectal carcinoma cells involves lysosomal membrane permeabilization and cathepsin D release”. Cell Death and Disease (2013) Feb 21;4:e507. doi: 10.1038/cddis.2013.29. Impact Factor: 5.378



Research Projects


Participation in USA project:

2016-ongoing- 3BP “Anticancer effect of 3-bromopyruvate (3BP) in animal models systems of leucemia- acronym 3BP”,(KoDiscovery, CEO Young Co), Baltimore  Maryland , United States USA.

Participation in National Research projects as team member:

2015/2017- Projeto financiado do CBMA como centro de excelência do qual é membro integrado -FCT I.P. através do financiamento estratégico CBMA-PEST 2015/2017 - UID/BIA/04050/2013. 

2016- 2018- Membro da equipa do WP2 do EcoAgriFood: “Innovative green products and processes to promote Agri-Food BioEconomy”, NORTE Regional Operational Program, Priority Axis I – Strenghening Research, Technological Development and Innovation, NORTE-45-2015-02, Structured R&D&I Projects.

Participation in European projects already concluded:

2013-2016- Team member on the Europeu Marie Curie Initial Training Networks (ITN): FP7-PEOPLE-2012-ITN - Marie Curie - proposal n° 317297 - acronym GLYCOPHARM.

2009-2013- European project FP7 NMP-2008-4.0-1, Acronym NANOFOL - Folate-based nanobiodevices for integrated diagnosis/therapy targeting chronic inflammatory diseases. Coordination: University of Minho, Braga, Portugal. Large Scale Collaborative Project. Coordinator: Artur Cavaco-Paulo, Department of Biological Engeneering, University of Minho. University of Minho was WP1 task leader with the following team: Ana Preto, Andreia Gomes, Artur Cavaco-Paulo.

Participated in the following national projects as a team member already concluded:

- “Identification of biological markers of diagnosis and prognosis in thyroid carcinomas, using “differential display” technique”, supported by FCT. (1995-1998) (PRAXIS XXI/2/2.1/SAU/1187/95).

- “Biological role of BRAF oncogene activation in human carcinogenesis. Use of BAY 43-9006 as a BRAF inhibitor agent?”, supported by BAYER in 2005 (drug suply).

- “Oncogenic role of BRAF activation in thyroid carcinogenesis”, supported by FCT. (2005-2008) (POCTI/SAU-OBS/56175/2004).

- “BRAF and KRAS mutations in mismatch repair deficiency colon cancer. New prognostic and therapeutic tool?”, supported by FCT. (2005-2008) (POCTI/SAU-OBS/56921/2004).

- “In vitro studies of compounds with putative anti-oxidant activity, obtained from medicinal plants traditionally used in Portugal”, supported by UNICER. (2005-2008).

- “Role of raft domains in the modulation/alteration of the transducing pathways in normal thyroid and in papillary thyroid carcinoma”, supported by FCT. (2004-2007).

- “Biological role of BRAF activation in colon carcinogenesis. STI-571/Gleevec as a therapeutic agent?”, supported by NOVARTIS. (2004 a 2006).

- “Potential mechanisms of tolerance to wild-type p53 in human tumours using differentiated thyroid carcinoma as a prototype”, supported by FCT (2003-2006). (Projecto POCTI/CBO/41084/2001).

- "Elucidation of ceramide-induced apoptosis: modulation of Protein Kinase C isoforms". Coordenation: Manuela Côrte-Real, Departamento de Biologia da Universidade do Minho, supported by FCT (2008-2012) (Project FCT PTDC/BIA-BCM/69448/2006).

- 2013-2016- Team member on the Project FCT-ANR/BEX-BCM/0175/2012: “Phosphoregulation of Bax-dependent cell death (Phosphobax)”. Coordination Manuela Côrte-Real, Department of Biology from the University of Minho.

Publications


Papers in scientific journals with peer review indexed in ISI:

· Zemzoumi K, Guilvard E, Sereno D, Preto A, Benlemlih M, Cordeiro da Silva A, Lemesre J, Ouaissi A. “Cloning of a Leishmania major gene encoding for an antigen with extensive homology to ribosomal protein S3a”. Gene, 240:57-65 (1999).

· Preto A, Reis Filho JS, Ricardo S, Soares P. “p63 expression in papillary and anaplastic carcinomas of the thyroid gland: lack of an oncogenic role in tumorigenesis and progression”. Pathology Research and Practice, 198: 449-454 (2002).

· Reis Filho JS, Preto A, Soares P, Ricardo S, Cameselle-Teijeiro J, Sobrinho-Simões M. “p63 in Solid cell nests: further evidence for a stem cell origin”. Modern Pathology, 16: 43-48 (2003).

· Reis Filho JS, Simpson PT, Martins A, Preto A, Gärtner F, Schmitt FC. “Distribution of p63, cytokeratin 5/6 and cytokeratin 14 in 51 normal and 400 neoplastic human tissue samples using TARP-4 multi-tumor tissue microarray.” Virchows Archiv, 443:122-132 (2003).

· Soares P, Trovisco V, Rocha A, Lima J, Castro P, Preto A, Máximo V, Botelho T, Seruca R and Sobrinho-Simões M. “BRAF mutations and RET/PTC rearrangements are alternative events in the etiopathogenesis of PTC”. Oncogene, 22:4578-80 (2003).

· Preto A, Singhrao S, Haughton M, Kipling D, Wynford-Thomas D and Jones CJ. “Telomere erosion triggers growth arrest but not cell death in human cancer cells retaining wild-type p53: implication for anti-telomerase therapy”. Oncogene, 23: 4136-45 (2004).

· Preto A, Cameselle-Teijeiro J, Moldes-Boullosa J, Soares P, Cameselle-Teijeiro JF, Silva P, Reis-Filho JS, Reyes-Santías RM, Alfonsín-Barreiro N, Forteza J, Sobrinho-Simões M. “Telomerase expression and proliferative activity suggest a stem cell role for thyroid solid cell nests”. Modern Pathology, 17:819-26 (2004).

· Máximo V, Preto A, Crespo A, Soares P, Machado JC, Rocha AS, Sobrinho-Simões M. “Core I gene is overexpressed in Hürthle and non-Hürthle cell microfollicular adenomas and follicular carcinomas of the thyroid”. BMC Cancer, 4:12 (2004).

· Máximo V, Botelho T, Capela J, Soares P, Lima J, Taveira A, Teresina A, Barbosa AP, Preto A, Rubén Harach R, Williams D, Sobrinho-Simões M. “Somatic and germline mutation in Grim-19, a dual function gene involved in mitochondrial metabolism and cell death, is linked to mitochondrion-rich (Hürthle cell) tumours of the thyroid”. Br J Cancer., 92:1892-8 (2005).

· Trovisco V, Soares P, Preto A, Vieira de Castro I, Lima J, Castro P, Máximo V, Botelho T, Moreira S, Meireles AM, Magalhães J, Abrosimov A, Cameselle-Teijeiro J, Sobrinho-Simões M: Type and prevalence of BRAF mutations are closely associated to papillary thyroid carcinoma histotype and patients’ age but not with tumour aggressiveness. Virchows Arch.,446:589-95 (2005).

· Cameselle-Teijeiro J, Preto A, Soares P , Sobrinho-Simões M. “A stem cell role for thyroid solid cell nests.” Human Pathology 36, 590– 593 (2005).

· Velho S, Oliveira C, Domingo E, Preto A, Hofstra RMW, Hamelin R, Yamamoto H, Seruca R, Schwartz S Jr.“ RASSF1A hypermethylation and concomintant KRAS/BRAF mutations occur preferentially in MSI sporadic colorectal cancer.” Oncogene, 24:7630-4 (2005).

· Sobrinho-Simões M, Preto A, Rocha AS, Castro P, Máximo V, Fonseca E, and Soares P “Molecular pathology of well-differentiated thyroid carcinomas.” Virchows Arch. 447:787-93 (2005).

· Oliveira C, Velho S, Moutinho C, Ferreira A, Preto A, Domingo E, Capelinha AF, Duval A, Hamelin R, Machado JC, Schwartz S Jr, Carneiro F, Seruca R. “KRAS and BRAF oncogenic mutations in MSS colorectal carcinoma progression.” Oncogene, 26:158-63 (2007).

· Preto A, Moutinho C, Rebocho AP, Velho S, Oliveira C, Figueiredo J, Lopes JM, Soares P and Seruca R. “A subset of colorectal carcinomas express c-KIT protein independently of KRAS and/or BRAF activation.” Virchows Archiv., 450:619-26 (2007).

· Sousa AM*, Preto A*, Rocha AS, Rebocho AP, Máximo V, Castro I, Moreira S, Botelho T, Trovisco V, Cirnes L, Alves C, Velho S, Soares P and Sobrinho-Simões M. “Molecular and genotypic characterization of human thyroid follicular-cell carcinoma derived cell lines”. Thyroid, 17:707-15 (2007). (* these authors contributed equally to the article)

· Trovisco V, Soares P, Preto A, Castro P, Máximo V, Sobrinho-Simões M. “Molecular genetics of papillary thyroid carcinoma – great expectations…”. Arq Bras Endocrinol Metab 51: 643-653 (2007).

· Preto A, Figueiredo J, Velho S, Ribeiro AS, Soares P, Oliveira C, Raquel Seruca. “BRAF provides proliferation and survival signals in MSI colorectal carcinoma cells displaying BRAFV600E but not KRAS mutations.” J Pathol. 214:320-7 (2008).

· Sobrinho-Simões M, Máximo V, Rocha AS, Trovisco V, Castro P, Preto A, Lima J and Soares P. “Intragenic Mutations in Thyroid Cancer”. Endocrinol Metab Clin North Am. 37:333-362 (2008).

· Xavier CPR, Lima CF, Preto A, Seruca R, Fernandes-Ferreira M and Pereira-Wilson C. “Luteolin, quercetin and ursolic acid are potent inhibitors of proliferation and inducers of apoptosis in both KRAS and BRAF mutated human colorectal cancer cells”. Cancer Lett. 281(2):162-70 (2009).

· Soares P, Preto A, Sobrinho-Simões M. “The BRAF V600E mutation in papillary thyroid carcinoma – a potential target for therapy?” Expert Rev. Endocrinol. Metab. 4(5), 467-480 (2009).

· Preto A, Gonçalves J, Rebocho AP, Figueiredo J, Meireles AM, Rocha AS, Trovisco V, Vasconcelos H, Seca H, Seruca R, Soares P, Sobrinho-Simões M. “Proliferation and survival molecules implicated in the inhibition of BRAF pathway in thyroid cancer cells harbouring different genetic mutations”. BMC Cancer. 9:387 (2009).

· Sousa MJ, Azevedo F, Pedras A, Marques C, Coutinho OP, Preto A, Gerós H, Chaves SR and Côrte-Real M. “Vacuole–mitochondrial cross-talk during apoptosis in yeast: a model for understanding lysosome–mitochondria-mediated apoptosis in mammals”. Biochem. Soc. Trans. 39, 1533–1537 (2011).

· Soares P, Lima J, Preto A, Castro P, Vinagre J, Celestino R, Couto J, Prazeres H, Eloy C, Máximo V, Sobrinho-Simões M. “Genetic alterations in poorly differentiated and undifferentiated thyroid carcinomas”, Current Genomics, 12( 8) , 609-617 (2011)

· Queirós O, Preto A, Pacheco A, Pinheiro C, Silva J, Moreira R, Pedro M, Ko Y, Pederson P, Baltazar F and Casal M. “Butyrate activates the monocarboxylate transporter MCT4 expression in breast cancer cells and enhances the antitumor activity of 3-bromopyruvate”. J Bioenerg Biomembr 2012, 44(1):141-53.

· Marques C, Oliveira CSF, Alves S, Chaves SR, Coutinho OP, Côrte-Real M, Preto A. “Acetate-induced apoptosis in colorectal carcinoma cells involves lysosomal membrane permeabilization and cathepsin D release.” Cell Death and Disease 2013, (2013) 4, e507; doi:10.1038/cddis.2013.29.

· Matamá T, Araújo R, Preto A, Cavaco-Paulo A and Gomes A. “In vitro induction of melanin synthesis and extrusion by tamoxifen”. International Journal of Cosmetic Science 2013 Apr 10. doi: 10.1111/ics.12052.

· Eugénia Nogueira, Ana Loureiro, Patrícia Nogueira, Jaime Freitas, Catarina R. Almeida, Johan Härmark, Hans Hebert, Alexandra Moreira, Alexandre M. Carmo, Ana Preto, Andreia C. Gomes, Artur Cavaco-Paulo. “Liposome and protein based stealth nanoparticles”. Faraday Discussions 2013, DOI: 10.1039/C3FD00057E

· João Vinagre, Ana Almeida, Helena Populo, Rui Batista, Joana Lyra, Vasco Pinto, Ricardo Coelho, Ricardo Celestino, Hugo Prazeres, Luis Lima, Miguel Melo, Adriana Rocha, Ana Preto, Patrícia Castro, Ligia Castro, Fernando Pardal, José Manuel Lopes, Lúcio Lara Santos, Rui Reis, José Cameselle-Teijeiro, Manuel Sobrinho-Simões, Jorge Lima, Valdemar Máximo, Paula Soares, “Frequency of TERT promoter mutations in human cancers”. Nature Communications 2013;4:2185. doi: 10.1038/ncomms3185.

 Baltazar F, Pinheiro C, Morais-Santos F, Azevedo-Silva J, Queirós O, Preto A, Casal M. “Monocarboxylate transporters as targets and mediators in cancer therapy response”. Histol Histopathol. 2014 Dec;29(12):1511-1524. Epub 2014 Jun 12.  Review. 

João Azevedo-Silva, Odilia Queirós, Fatima Baltazar, Young H. KO, Peter Pedersen, Ana Preto, Margarida Casal. “The cytotoxicity of 3-Bromopyruvate in breast cancer cells depends on extracellular pH”.  Biochem J. 2015 Apr 15;467(2):247-58. doi: 10.1042/BJ20140921.

Ana Loureiro, Eugénia Nogueira, Nuno G. Azoia, Marisa Passos Sárria, Ana Sofia Abreu, Ulyana Shimanovich, Alexandra Rollett, Johan Härmark, Hans Hebert, Georg Guebitz, Gonçalo J. L. Bernardes, Ana Preto, Andreia C. Gomes, Artur Cavaco-Paulo. “Size Controlled Protein Nanoemulsions for Active Targeting of Folate Receptor Positive Cells”. Colloids and Surfaces B: Biointerfaces, 2015, 135, 90-98. doi:10.1016/j.colsurfb.2015.06.073;

Ana Loureiro, Gonçalo J. L. Bernardesc, Ulyana Shimanovichc, Marisa Passos Sárriaa,b, Eugénia Nogueira, Ana Preto, Andreia C. Gomesb, Artur Cavaco-Paulo. “Folic Acid-Tagged Protein Nanoemulsions Loaded with CORM-2 Enhance the Survival of Mice Bearing Subcutaneous A20 Lymphoma Tumors”, Nanomedicine. 2015 Jul;11(5):1077-83. doi: 10.1016/j.nano.2015.02.022. Epub 2015 Mar 16. 2014.

Eugénia Nogueira, Franck Lager, Delphine Le Roux, Patrícia Nogueira, Jaime Freitas, Celine Charvet, Gilles Renault, Ana Loureiro, Catarina R. Almeida, Anna Ohradanova-Repic, Christian Machacek, Gonçalo J. L. Bernardes, Alexandra Moreira, Hannes Stockinger, Michael Burnet, Alexandre M. Carmo, Andreia C. Gomes, Ana Preto, Georges Bismuth and Artur Cavaco-Paulo. "Enhancing methotrexate tolerance with folate tagged liposomes in arthritic mice." Journal of Biomedical Nanotechnology, 2015, 11(12), 2243-2252.

Oliveira CSF, Pereira H, Alves S, Castro L, Baltazar F, Chaves SR, Côrte-Real M #, Preto A #. "Cathepsin D protects colorectal cancer cells from acetate-induced apoptosis through autophagy-independent degradation of damaged mitochondria".  # co-senior authorship. Cell Death Dis. 2015 Jun 18;6:e1788. doi: 10.1038/cddis.2015.157. 2014.

H. Pereira, C.S.F. Oliveira, L. Castro, A. Preto, S. R. Chaves, M. Côrte-Real. “Yeast as a tool to explore cathepsin D function”. Microbial Cell, July 2015 | Vol. 2 No.7 doi: 10.15698/mic2015.07.212

Amorim R, Pinheiro C, Miranda-Gonçalves V, Pereira H, Moyer MP, Preto A, Baltazar F. “Monocarboxylate transport inhibition potentiates the cytotoxic effect of 5-fluorouracil in colorectal cancer cells.” Cancer Lett. 2015 Aug 28;365(1):68-78. doi: 10.1016/j.canlet.2015.05.015. Epub 2015 May 25.  2014.

Alves S, Castro L, Fernandes MS, Francisco R, Castro P, Priault M, Chaves SR, Moyer MP, Oliveira C, Seruca R, Côrte-Real M, Sousa MJ, Preto A. “Colorectal cancer-related mutant KRAS alleles function as positive regulators of autophagy”. Oncotarget. 2015 Oct 13;6(31):30787-802. doi: 10.18632/oncotarget.5021

Nogueira E., MangialavoriI. C., Loureiro A., Azoia N. G., Sárria M. P., Nogueira P., Freitas J., Harmark, J., Shimanovich U., Rollett A., Lacroix G., Bernardes G. J. L., Guebitz G., Hebert H., Moreira A., Carmo A. M., Rossi J. P. F. C., Gomes A. C., Preto A. and Cavaco-Paulo A. “Peptide anchor for folate-targeted liposomal delivery”. Biomacromolecules, 2015, 16(9), 2904-2910.

Castro L, Alves S, Chaves SR, Costa JL, Soares P#, Preto A#. “ The role of RAF-1 in thyroid cancer cells signaling and survival. “RAF-1 promotes survival of thyroid cancer cells harboring RET/PTC1 rearrangement independently of ERK activation”. # co-senior authorship Mol Cell Endocrinol. 2015 Nov 5;415:64-75. doi: 10.1016/j.mce.2015.08.006. 

Eugénia Nogueira, Andreia Gomes, Ana Preto and Artur Cavaco-Paulo. “Design of Liposomal Formulations for Cell Targeting”. Colloids Surf B Biointerfaces. 2015 Dec 1;136:514-26. doi: 10.1016/j.colsurfb.2015.09.034. Epub 2015 Sep 25. Review.

Eugénia Nogueira, Andreia Gomes, Ana Preto and Artur Cavaco-Paulo. “Folate-targeted nanoparticles for rheumatoid arthritis therapy.” Nanomedicine. 2015 Dec 28. pii: S1549-9634(15)00610-3. doi: 10.1016/j.nano.2015.12.365. [Epub ahead of print] Review.

Nogueira E, Cruz CF, Loureiro A, Nogueira P, Freitas J, Moreira A, Carmo AM, Gomes AC, Preto A, Cavaco-Paulo A.. “Assessment of nanocapsule disruption to drug delivery in vitro”. Biochim Biophys Acta. 2016 Feb;1858(2):163-7. doi: 10.1016/j.bbamem.2015.11.008. Epub 2015 Nov 14.

Nogueira E., Gomes A. C., Preto A. and Cavaco-Paulo A.  “Update on therapeutic approaches for rheumatoid arthritis.” Curr Med Chem. 2016;23(21):2190-203.

Martins SF, Amorim R, Viana-Pereira M, Pinheiro C, Costa RF, Silva P, Couto C, Alves S, Fernandes S, Vilaça S, Falcão J, Marques H, Pardal F, Rodrigues M, Preto A, Reis RM, Longatto-Filho A, Baltazar F. “Significance of glycolytic metabolism-related protein expression in colorectal cancer, lymph node and hepatic metastasis”, BMC Cancer. 2016 Jul 26;16:535. doi: 10.1186/s12885-016-2566-9.

Ferro S, Azevedo-Silva J, Casal M, Côrte-Real M, Baltazar F, Preto A. “Characterization of acetate transport in colorectal cancer cells and potential therapeutic implications”. Oncotarget. 2016 Sep 21. doi: 10.18632/oncotarget.12156. IF 2015: 6.359

Azevedo-Silva J., Ko Y. H., Pedersen P. L., Preto A., Casal M. “Anticancer agent 3BP: license to kill. “3-Bromopyruvate: from a simple molecule to the bedside of oncology patients”. J Bioenerg Biomembr. 2016 Aug;48(4):349-62. doi: 10.1007/s10863-016-9670-z

Nogueira E, Gomes AC, Preto A, Cavaco-Paulo A. “Folate-targeted nanoparticles for     rheumatoid arthritis therapy”. Nanomedicine. 2016 May;12(4):1113-26. doi: 10.1016/j.nano.2015.12.365.

Eugénia Nogueira, Ana Loureiro, Patrícia Nogueira, Jaime Freitas, Franck Lager, Delphine Le Roux, Celine Charvet, Gilles Renault, Georges Bismuth, Alexandra Moreira, Alexandre M. Carmo, Andreia C. Gomes, Ana Preto, Artur Cavaco-Paulo. Neutral PEGylated liposomal formulation for efficient folate-mediated delivery of MCL1 siRNA to activated macrophages. Colloids and Surfaces B: Biointerfaces, in press.

 

 

 

Abstracts published in proceedings of scientific meetings

Reis Filho JS, Preto A, Ricardo S, Soares P. “p63 expression in papillary and anaplastic carcinomas of the thyroid gland”. XXIVth International Congress of the International Academy of Pathology. Amesterdan (Holand), October 2002. [Abstract published in: Histopathology, 41 (suplement 1):186, (P.16.248), 2002].

Reis Filho JS, Preto A, Soares P, Ricardo S, Cameselle-Teijeiro J, Sobrinho-Simões M.“p63 in Solid cell nests: further evidence for a stem cell origin”. XXIVth International Congress of the International Academy of Pathology. Amesterdan (Holand), October 2002. [Abstract published in: Histopathology, 41 (suplemento 1):56-57, (O.16.248), 2002].

Reis-Filho JS, Martins A, Preto A, Soares P, Schmitt FC. “Distribution of p63, Cytokeratin 5/6 and Cytokeratin 14 in 51 Normal and 450 Neoplastic Human Tissue Samples Using TARP-4 Multi-Tumor Tissue Microarray”. 92th Annual Meeting of the United States and Canadian Academy of Pathology. Washington DC (USA), March 2003. [Abstract published in: Modern Pathology, 16: 1493, 2003.].

Preto A, Haughton M, Kipling D, Soares P, Wynford-Thomas D and Jones CJ. “P53 mediated growth arrest in response to telomere erosion is retained in a subset of human cancer cell lines.” 1st Meeting of the Portuguese Society of Pharmaceutical Sciences. Lisbon (Portugal), April 2003. [Abstract published in: Revista Portuguesa de Farmácia, vol LII, (nº1), suplement: 16, (O-8), 2003].

Preto A, Rebocho AP, Meireles AM, Seruca R, Soares P, Sobrinho-Simões M. “Novas terapêuticas no carcinoma da tireóide: efeito biológico da inibição farmacológica de cinases em linhas celulares do carcinoma da tireóide”. 7th National Symposium of Endocrine Tumours, Porto, February 2007. [Abstract published in: Revista Oncologia, Special edition, February 2007].

Xavier CPR, Lima CF, Preto A, Seruca R, Fernandes-Ferreira M and Pereira-Wilson C. Quercetin, Luteolin and ursolic acid are potent inhibitors of proliferation in colorectal carcinoma cells: new therapeutic tools? (2008) [Abstract published in: European Journal of Cancer Supplements, 6 (9): 140].

Ferreira M, Araújo R, Preto A, Gomes AC, Cavaco-Paulo A (2009). Capillary treatments using siRNA oligonucleotides- a new gene therapy tool? 14th Annual Meeting, EHRS, European Hair Research Society, Graz, Austria, July 2-4, 2009. [Abstract published in: Int J Trichol;1:60-74].

A Pacheco , O Queirós , A Preto , C Pinheiro , J Azevedo-Silva , R Moreira , M Pedro , F Baltazar , M Casal. 3-bromopyruvate cytotoxic effect in breast cancer cells is dependent of monocarboxylate transporters (MCT) expression and is enhanced by butyrate. Exp Clin Endocrinol Diabetes 2012; 120 - A22. DOI: 10.1055/s-0032-1330817

Sousa, MJ ; Azevedo, F; Pedras, A; Marques, C; Coutinho, OP ; Preto, A; Geros, H ; Chaves, SR; Corte-Real, M. Vacuole-mitochondrial cross-talk during apoptosis in yeast: a model for understanding lysosome-mitochondria-mediated apoptosis in mammals (vol 39, pg 1533, 2011). BIOCHEMICAL SOCIETY TRANSACTIONS Volume: 39 Pages: 1901-1901 Part: 6 Published: DEC 2011

R. Amorim, N. Vilaca, A.M. Fonseca, I.C. Neves, A. Preto, F. Baltazar. Enhancing 5-FU Activity in Colorectal Carcinoma-derived Cell Lines – Combination With Monocarboxylate Transporter Inhibitors and Encapsulation into Zeolites. European Journal of Cancer. Volume 48, Supplement 5, July 2012, Pages S100. 22nd Biennial Congress of the European Association for Cancer Research

Nogueira, ES, Preto, A; Cavaco-Paulo, A. Liposomal formulations for specific drug delivery. ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY Volume: 243 Meeting Abstract: 671-COLL, Published: MAR 25 2012 .

Azevedo-Silva J, Queirós O, Ko YH, Pedersen P, Preto A and Casal M (2014). Characterization of 3-bromopyruvate uptake in breast cancer cells. Front. Pharmacol. Conference Abstract: 4th Annual Meeting of the International Society of Proton Dynamics in Cancer. doi:10.3389/conf.fphar.2014.61.00003.

Matos A, Marques F, Francisco R, Moreira T, Preto A, Garcia MH, Valente A. “Unraveling the mode of action of new promising polymer-ruthenium conjugates”. Ultrastruct Pathol. 2017 Jan-Feb;41(1):129-130. doi: 10.1080/01913123.2016.1272669.


Giulia Cazanelli, Suellen Ferro, Tiago Moreira, João Silva, Eugenia Nogueira, Ana Preto. Colorectal cancer therapeutic approaches: from classical drugs to new nanoparticles. Frontiers in Anti-Cancer Drug Discovery, 2016, Vol7, 3-82. Chapter3. Bentham eBooks, Bentham Science Publishers.


- Cavaco Paulo A., Gomes A., Marques, R., Loureiro A., Preto A., Universidade do Minho. WO2012IB57082: Formulations for micelle formation comprising a protein and methods preparation thereof. Priority: PT20110106047 2011/12/07.

-Cavaco Paulo A., Preto A., Nogueira E., Gomes A., Universidade do Minho. WO2012IB57083: Liposomes and method for producing the same. Priority: PT20110106050 2011/12/07.

 

Scientific Comunications (83)

Oral communications (24)

- Reis Filho JS, Preto A, Soares P, Ricardo S, Cameselle-Teijeiro J, Sobrinho-Simões M.“p63 in Solid cell nests: further evidence for a stem cell origin”. XXIVth International Congress of the International Academy of Pathology. Amesterdan (Holand), October 2002.

- Preto A, Haughton M, Kipling D, Soares P, Wynford-Thomas D and Jones CJ. “P53 mediated growth arrest in response to telomere erosion is retained in a subset of human cancer cell lines.” 1º Congresso da Sociedade Portuguesa de Ciências Farmacêuticas. Lisbon (Portugal), Abril 2003.

- Preto A, Rebocho AP, Meireles AM, Seruca R, Soares P, Sobrinho-Simões M. “Novas terapêuticas no carcinoma da tireóide: efeito biológico da inibição farmacológica de cinases em linhas celulares do carcinoma da tireóide”. “Tumores endócrinos-7º simpósio nacional”, Porto, February 2007.

- Preto A, Haughton M, Singhrao S, Kipling D, Wynford-Thomas D and Jones CJ. “P53 mediated growth arrest in response to telomere erosion is retained in a subset of human cancer cell lines”. European Thyroid Association Annual Meeting. Edimburgo, Escócia (UK), October de 2003.

- Preto A, Rebocho AP, Meireles AM, Seruca R, Soares P and Sobrinho-Simões M. “Biological effects of kinase inhibition in thyroid cancer cell lines using a pharmacological approach.”  Thyroid meeting, IPATIMUP, Porto (Portugal), March 2004.

- Preto A, Rebocho AP, Gonçalves J, Meireles AM, Figueiredo J, Rocha AS, Trovisco V, Vasconcelos MH, Seca H, Seruca R, Soares P, Sobrinho-Simões M. “SENSITIVITY OF THYROID CANCER CELLS TO BAY 43-9006 IS DISTINCT FROM BRAF SPECIFIC INHIBITION. BIOLOGICAL AND THERAPEUTIC IMPLICATIONS”. “First Iberic Meeting on Medical Chemistry-Anticancer Agents”, Régua, April de 2007.

- Velho S, Preto A, Figueiredo J, Oliveira C, Seruca R.“MAP kinase signalling pathway in gastrointestinal cancer”. “First Iberic Meeting on Medical Chemistry-Anticancer Agents”, Régua, April de 2007.

- KRAS and BRAF mutations in colorectal cancer progression. Velho S, Oliveira C, Preto A, Capelinha AF, Hamelin R, Schmitt F, Carneiro F, and Seruca R. “Porto Cancer meeting”, April 2008.

- Alves S , Chaves S , Oliveira C, Seruca R  , Côrte-Real M , Preto A  and Sousa MJ  “A yeast heterologous expression system to understand the role of human oncogene KRAS in autophagy regulation”. “Jornadas Portuguesas de Genética” 31 de May, 1 e 2 de June de 2010, Braga.

- Chaves S, Pereira C, Marques C, Rodrigues A, Salin B, Alves S, Silva R, Gerós H, Coutinho O, Preto A, Camougrand N, Manon S, Sousa MJ and Côrte-Real M. “What yeast can tell us about how cells commit suicide?” “14th Portugaliae Genetica”, 17-18 March 2011, Porto.

- “Vacuole-mitochondrial crosstalk during acetic acid-induced yeast apoptosis.” Chaves S, Pereira C, Marques C, Rodrigues A, Salin B, Alves S, Silva R, Gerós H, Coutinho O, Preto A, Camougrand N, Manon S, Sousa MJ and Côrte-Real M. 8th International Meeting on Yeast Apoptosis, 2-6 May 2011, Canterbury, England.

- “Liposomal formulations for specific drug delivery”. Nogueira E, Preto A, Cavaco-Paulo A- ACS,  Abril 2012, São Diego.

 - “Discovering messenger RNA and micro RNA expression patterns in normal, greying hair.” Araújo R, Matamá T, Preto A, Cavaco-Paulo A e Gomes A. European Hair Research Society, Barcelona, June 2012.

- Eugenia Nogueira, Ana Preto, Artur Cavaco Paulo. New peptide-linker of folate-target liposomes for specific drug delivery. 246th ACS Meeting, 8-12 September 2013, Indianopolis, USA.

- Azevedo-Silva,J. , Queirós, O., Ko, Y.H., Pedersen, P.L., Preto, A. ,Casal, M.  Characterization of 3-bromopyruvate uptake in breast cancer cells.  The 4th Annual Meeting of the International Society of Proton Dynamics in Cancer, Munich, 2013.

- C.S.F. Oliveira, H. Pereira, S. Alves, S. R. Chaves, M.J. Sousa, A. Preto, M. Côrte-Real. “The yeast vacuolar protease Pep4p unveils a novel function of the human Cathepsin D during acetate-induced mitochondrial apoptosis in colorectal carcinoma cells”. IMYA X INTERNATIONAL MEETING ON YEAST APOPTOSIS, May 14th -May 18th 2014, Chalmers University of Technology, Gothenburg, Sweden.

- Eugénia Nogueira, Preto A, Cavaco-Paulo A. “Characterization of peptides as a membrane anchor in liposomes for the incorporation of ligands for specific targeting”. IPFB2014 - 8th International Conference on Polymer and Fiber Biotechnology - 25-27 May 2014, Braga, Portugal.

- A. Valente, M.H. Garcia, A. Pilon, A.P. Matos, F. Tortosa, N. Mendes, F. Gärtner, A. Preto, R. Francisco, B. T. Buckley, F. Marques "On the mode of action of new promising anticancer agents: multifunctional polymer-ruthenium conjugates", , 4th International Conference on Medicinal Chemistry & Computer Aided Drug Designing, November 02-04, 2015 Atlanta, USA.

- A. Valente, M.H. Garcia, A. Pilon, A.P. Matos, F. Mendes, M.A.F. Faustino, N. Mendes, F. Gärtner, A. Preto, R. Francisco, F. Marques, "Multifunctional polymer-ruthenium cyclopentadienyl complexes: new promising anticancer agents", I International Caparica Christmas Congress on Translational Chemistry 7th-10th December 2015, Portugal –

-  Sara Alves, Lisandra Castro, Maria Sofia Fernandes, Rita Francisco, Paula Castro, Muriel Priault, Susana Rodrigues Chaves, Mary Pat Moyer, Carla Oliveira, Raquel Seruca, Manuela Côrte-Real, Maria João Sousa, Ana Preto. Oral flash presentation. “Colon cancer-related mutant KRAS alleles function as positive regulators of autophagy,  IMYA 29 setembro-3 outubro 2015, Porto.

- Azevedo-Silva J., Oliveira C.S.F., Queirós O.,Ribas D., Côrte-Real M., Baltazar F., Goffeau A., Ko Y.H., Pedersen P.L., Preto A., Casal M., “Transport of monocarboxylic acids in cancer cells: implications of short chain fatty acids and 3-bromopyruvate for cancer therapy”. 34th SMYTE, Small meeting on yeast transport and energetics, Chania (Greece), 29th August- 1st september 2016.

- Andreia Valente, Leonor Côrte-Real, Tiago Moreira, Ana Preto, M. Helena Garcia. “Targeting cancer with new multifunctional metallodrugs”. World Conference on Cancer Research and Therapy, Miami, USA, nov 2016.

- Azevedo-Silva J., Oliveira C.S.F., Queirós O.,Ribas D., Côrte-Real M., Baltazar F., Goffeau A., Ko Y.H., Pedersen P.L., Preto A., Casal M. “Transport of short chain fatty acids and 3-bromopyruvate in cancer cells: implications for cancer therapy”. Sociedade Portuguesa de Bioquimica (SPB), Guimarães, 8-10 dez 2016.

 

 

Posters (58)

- Reis Filho JS, Preto A, Ricardo S, Soares P. “p63 expression in papillary and anaplastic carcinomas of thethyroid gland”. XXIVth International Congress of the International Academy of Pathology. Amesterdan (Holand), October 2002. [Abstract publicado na: Histopathology, 41 (suplemento 1):186, (P.16.248), 2002].

- Reis-Filho JS, Martins A, Preto A, Soares P, Schmitt FC. “Distribution of p63, Cytokeratin 5/6 and Cytokeratin 14 in 51 Normal and 450 Neoplastic Human Tissue Samples Using TARP-4 Multi-Tumor Tissue Microarray”. 92th Annual Meeting of the United States and Canadian Academy of Pathology. Washington DC (USA), March 2003. [Abstract publicado na: Modern Pathology, 16: 1493, 2003.].

- Preto A, Jones CJ, Kipling D, Soares P, Wynford-Thomas D e Sobrinho-Simões M: “Efeito da inibição da telomerase em linhas celulares de carcinoma papilar da tireóide”. 5ª Reunião da Sociedade Portuguesa de Genética Humana. Aveiro (Portugal), October 2001.

- Preto A, Kipling D, Soares P, Wynford-Thomas D and Jones CJ..”Telomerase inhibition in cancer cells retaining wild-type p53”. XIIth EuroCellPath Course- Cancer as a Disease of Genes and Gene Regulation. Berlim (Alemanha), May 2002.

- Preto A, Haughton M, Mehle C, Singhrao S., Kipling D, Soares P, Wynford-Thomas D and Jones CJ..”Telomerase inhibition in cancer cells retaining wild-type p53”. Cancer Genetics and Tumor Suppressor Genes Meeting. Cold Spring Harbor. Nova York (USA), August de 2002.

- Preto A, Haughton M, Kipling D, Wynford-Thomas D and Jones CJ. “P53 mediated growth arrest in response to telomere erosion is retained in a subset of human cancer cell lines”. American Association for Cancer Research Special Meeting: “Telomeres, telomerase and cancer.” San Francisco (USA), December 2002.

- Rebocho AP, Trovisco V, Castro P, Preto A, Meireles AM, Rocha AS, Lima J, Moreira S, Feijão T, Botelho T, Máximo V, Soares P. “RAF-1 in contrast to BRAF is not a mutational target of thyroid carcinogenesis”. XIII Porto Cancer Meeting- Mismatch repair genes, Microsatellite instability, and Cancer, IPATIMUP, Porto, Portugal, May 2004.

- Velho S, Oliveira C, Cirnes L, Preto A, Hofstra RMW, Hamelin R, Seruca R. “RASSF1A methylation analysis in a series of colorectal cancer cell lines: ongoing work”. XIII Porto Cancer Meeting- Mismatch repair genes, Microsatellite instability, and Cancer, IPATIMUP, Porto, Portugal, May 2004.

- Preto A, Figueiredo ,J Velho S, Ribeiro AS, Soares P, Oliveira C, Seruca R. BRAF provides proliferation and survival signals in MSI colorectal carcinoma cells displaying BRAFV600E but not KRAS mutations. “11ª Reunião da Sociedade Portuguesa de Genética Humana”, Porto, November 2007.

- Xavier CPR, Lima CF, Preto A, Seruca R, Fernandes-Ferreira M and Pereira-Wilson C. “Effects of natural compounds in PI3K/AKT and MAPK/ERK pathway on human colorectal cell lines” Sinal 2008: Crosstalk Between Signaling Pathways". Second National Meeting of Signal Transduction. IBMC, Porto, Portugal, May 16-17, 2008.

- Ferreira M, Araújo A, Preto A, Gomes AC, Cavaco-Paulo A. “Capillary treatments using siRNA oligonucleotides- a new gene therapy tool?”. 14th Meeting of the European Hair Research Society, Graz, Austria, July 2-4, 2009.

- Preto A, Gonçalves J, Rebocho AP, Figueiredo J, Meireles AM, Rocha AS, Trovisco V, Vasconcelos H, Seca H, Seruca R, Soares P, Sobrinho-Simões M. “Proliferation and survival molecules implicated in the inhibition of BRAF pathway in thyroid cancer cells harbouring different genetic mutations”. Annual Meeting of the European Thyroid Association, Lisbon, Portugal, September 5-9, 2009.

- Nogueira E., Ferreira H., Preto A. and Cavaco-Paulo A. “Characterization of several liposomal formulations for methotrexate delivery “. 239th American Chemical Society National Meeting & Exposition, San Francisco, CA (EUA), 21-25 March 2010.

- Marques C, Côrte-Real M, Coutinho OP, Preto A. “Response to acetate in colorectal carcinoma cells harboring different genetic backgrounds”. “Jornadas Portuguesas de Genética” 31 de May, 1 e 2 de June de 2010, Braga.

- Marques C, Côrte-Real M, Coutinho OP, Preto A. “The involvement of the lysosome in death induced by acetate in colorectal carcinoma cells harboring different genetic backgrounds”. XVII “Congresso Nacional de Bioquimica”, 15- 17 December 2010, Porto.

- Alves S, Chaves S, Oliveira C, Seruca R , Côrte-Real M, Preto A and Sousa MJ. “The role of human oncogene KRAS in autophagy regulation: Saccharomyces cerevisiae as a model system”. “XVII Congresso Nacional de Bioquimica”, 15- 17 December 2010, Porto.

- Pires S, Queiroz MJRP, Preto A and Coutinho OP. “Effect of two di(hetero)arylamines raloxifen-like in proliferation and apoptosis of breast cancer cells”. “XVII Congresso Nacional de Bioquimica”, 15- 17 December 2010, Porto.

- Amorim R, Pereira H, Preto A, Baltazar F. “Monocarboxylate transporters – new molecular targets for colorectal carcinoma?”. XX Porto Cancer Meeting 28-29 April 2011, Porto.

-  Pires S, Silva E, Queiroz MJRP, Preto A  and Coutinho OP. “NEW SYNTHETIC DI(HETERO)ARYLAMINES RALOXIFEN-LIKE INDUCE APOPTOSIS OF BREAST CANCER CELLS AND SPECIFICALLY BIND TO ESTROGEN RECEPTOR”. "4th International Symposium on Advances in Synthetic and Medicinal Chemistry" (Agosto, 2011) August 21-25, 2011, St. Petersburg, Russia.

- Amorim R, Martins S, Pereira H, Pinheiro C, Reis R, Longatto-Filho A, Preto A, Baltazar F. "Monocarboxylate transporters – new molecular targets for colorectal carcinoma?”. Zing Cancer Conference 2011: from carcinogenesis to cancer therapy; 9-13 November (2011), Xcaret, Mexico.

- “Monocarboxylate transporters as new attractive targets for colorectal carcinoma therapy”. Amorim R, Martins S, Pereira H, Pinheiro C, Reis R, Longatto-Filho A, Preto A, Baltazar F. 2nd Symposium of the International Society for Proton Dynamics in Cancer (ISPDC): Basic Research to Cancer Treatment; 18-19 November (2011), Nice, France.

-“Enhancing 5-FU activity in colorectal carcinoma-derived cell lines: combination with monocarboxylate transporter inhibitors and encapsulation into zeolites”. Amorim R, Vilaça N, Fonseca AM, Neves I C, Preto A, Fátima Baltazar. EACR, Barcelona 2012.

- “Butyric acid increases monocarboxylate transporters (MCT) expression and enhances the cytotoxicity of the anti-tumoral agent 3-bromopyruvate (3-BP) in breast cancer cells”. Pacheco A, Queirós O, Preto A, Pinheiro C, Silva JA, Moreira R, Pedro M, Baltazar F and Casal M. Porto Cancer Meeting, 19, 20 Abril, 2012.

-  “The role of KRAS mutations in autophagy regulation in colorectal carcinoma”.Alves S, Chaves S R, Oliveira C, Seruca R, Corte-Real M, Sousa MJ, Preto A. Porto Cancer Meeting, Porto 19,20 Abril, 2012.

- “The role of lysosomal membrane permeabilization in acetate-induced apoptosis in colorectal carcinoma cells”. Marques C , Oliveira CSF , Alves S, Côrte-Real M, Coutinho OP, Preto A. Porto Cancer Meeting, Porto 19,20 Abril, 2012.

- “Combination of 5-FU with monocarboxylate transporters inhibitors as a new therapeutic approach in colorectal carcinoma”. Amorim R, Pereira H, Preto A, Baltazar F. Porto Cancer Meeting, Porto 19,20 Abril, 2012.

- “Tamoxifen speeds up melanin extrusion in normal human melanocytes.” Matamá T, Araújo R, Preto A, Gomes A, Cavaco-Paulo A. European Hair Research Society, Barcelona, June 2012.

- “Butyric acid increases monocarboxylate transporters (MCT) expression and enhances the cytotoxicity of the anti-tumoral agent 3-bromopyruvate (3-BP) in breast cancer cells”. Pacheco A, Queirós O, Preto A, Pinheiro C, Azevedo-Silva J, Moreira R, Pedro M, Baltazar F and Casal M. “Jornadas Portuguesas de Genetica”, Coimbra, Abril 2012.

-“Using zeolites as systems for drug delivery in in vitro models of colorectal carcinoma”. Amorim R, Vilaça N, Preto A, Fonseca AM, Neves IC, Baltazar F. II ICVS/3B meeting, Braga, Junho 2012.

- “Transport of lactic acid across plasma membrane: implications in health and biotechnology”. Casal M, Pacheco A, Sá-Pessoa J, Azevedo-Silva J, Preto A, Pinheiro C, Ko YH, Pedersen PL, U?aszewski S, Goffeau A, Baltazar F, Paiva S, Queirós O. SMYTE 30, 9-12 July 2012, Salamanca, Spain.

- “Unravel the role of KRAS in autophagy control in colorectal carcinoma: Saccharomyces cerevisiae as a model system”. Alves S , Chaves SR, Oliveira C , Seruca R , Corte-Real M , Preto A, Sousa MJ.  IMYA 2012 9th International Meeting on Yeast Apoptosis, 16-20th September 2012, Italy.

- Castro L, Soares P, Preto A. “Study of the role of RAF-1 in MAPK signaling in thyroid cancer cells”. Porto Cancer Meeting, Porto 11,12 Abril, 2013.

- Eugénia Nogueira, Ana Preto, Artur Cavaco-Paulo. Characterization of a new peptide as membrane anchor for the incorporation of ligand targeting into liposomes. Abstract Faraday Discussion 166: Self-Assembly of Biopolymers, 16-18 September 2013, University of Bristol ,UK

- Oliveira, C. S.F. ; Marques, C. ; Alves, S; Chaves, SR ; Coutinho, OP ; Côrte-Real, M ; Preto, A. Involvement of lysosomal membrane permeabilization and cathepsin D release in colorectal carcinoma cells acetate-induced apoptosis. 1st PDBMA symposium, I Jornadas do Programa Doutoral em Biologia Molecular e Ambiental, Braga, 28 novembro 2013.

- C. S.F. Oliveira, C. Marques, S. Alves, S. Chaves, M. Côrte-Real, A. Preto. Acetate induces lysosomal membrane permeabilization, cathepsin D release and impairs autophagy in colorectal carcinoma cells. 2nd EACR Special Conference on Cell Death in Cancer. Amsterdam, Netherlands, 30 January –1 February 2014.

 

 

- Marta A. Casanova, Ana Preto, Lígia R. Rodrigues. "Improved production of acetate and propionate by Propionibacterium freudenreichii”. XVIII Congress of the Portuguese Biochemical Society (SPB), December 17-20, 2014.

- C.S.F. Oliveira, H. Pereira, S. Alves, S. R. Chaves, A. Preto, M. Côrte-Real. "Cathepsin D protects colorectal carcinoma cells from acetate-induced apoptosis through autophagy-independent degradation of damaged mitochondria: a new function unveiled by the yeast vacuolar protease Pep4p”. XVIII Congress of the Portuguese Biochemical Society (SPB), December 17-20, 2014.

- J. Azevedo-Silva, O. Queirós, A. Ribeiro, F. Baltazar, Y. H. Ko, P. L. Petersen, A. Preto & M. Casal. “The cytotoxicity of 3-Bromopyruvate in breast cancer cells depends on extracellular pH”. 10th European Biophysics Congress , July 18 -22, 2015, Dresden, Germany.

-  Sara Alves, Lisandra Castro, Maria Sofia Fernandes, Paula Castro, Muriel Priault, Susana Rodrigues Chaves, Mary Pat Moyer, Carla Oliveira, Raquel Seruca, Manuela Côrte-Real, Maria João Sousa, Ana Preto. Hotspot mutations in the KRAS oncogene function as positive regulators of autophagy in the colon. XXIII Porto Cancer Meeting - Stem Cells and Cancer, 7-8 may 2015, Porto.

- Suellen Ferro, Helena Pereira, Sara Alves , Lisandra Castro, Fátima Baltazar, Susana Chaves, Manuela Côrte-Real, Ana Preto. “Cathepsin D is involved in the degradation of damaged mitochondria during acetate-induced apoptosis in colorectal cancer cells”. XXIII Porto Cancer Meeting - Stem Cells and Cancer, 7-8 may 2015, Porto.

- Eugénia Nogueira, Artur Cavaco-Paulo, Ana Preto. “Folate-target liposomal nanoparticles for cancer therapy”. XXIII Porto Cancer Meeting - Stem Cells and Cancer, 7-8 may 2015, Porto.

- Marta Casanova, Lígia Rodrigues  and Ana Preto . “Short chain fatty acids produced by Propionibacterium freudenreichii inhibit colorectal cancer cells survival”. Porto Cancer Meeting - Stem Cells and Cancer, 7-8 may 2015, Porto. XXIII Porto Cancer Meeting - Stem Cells and Cancer, 7-8 may 2015, Porto.

- Sara S. Nogueira, Gerald Brezesinski, Andreia C. Gomes, Ana Preto, M. Elisabete C. D. Real Oliveira. “Monoolein-based nanocarriers for siRNA delivery in colorectal cancer therapy.” 6th Iberian Meeting on Colloids and Interfaces (RICI6), 8- 10 of July 2015, Guimarães, Portugal.

- Azevedo-Silva J., Baltazar F., Ko Y. H., Petersen P. L., Preto A., Casal M. “Silencing of Monocarboxylate transporters as a tool to study the role of 3-bromopyruvate in breast cancer”. XXXIX Jornadas Portuguesas de Genética, 25- 27 de Maio de 2015, Campus de Gualtar da Universidade do Minho, Braga.

- Suellen Ferro, Helena Pereira, Sara Alves, Lisandra Castro, Fátima Baltazar, Susana Chaves, Ana Preto and Manuela Côrte-Real. “Cathepsin D protects colorectal cancer cells from acetate-induced apoptosis through autophagy-independent degradation of damaged mitochondria”. XXXIX Jornadas Portuguesas de Genética, 25- 27 de Maio de 2015, Campus de Gualtar da Universidade do Minho, Braga.

-  Marta Casanova 1,2 *, Lígia Rodrigues and Ana Preto. Propionibacterium freudenreichii short-chain fatty acids inhibit the proliferation of colorectal cancer cells. XXXIX Jornadas Portuguesas de Genética, 25- 27 de Maio de 2015, Campus de Gualtar da Universidade do Minho, Braga.

- Cláudia Suellen F. Oliveira, Helena Pereira, Sara Alves, Lisandra Castro, Fátima Baltazar, Susana R. Chaves, Ana Preto, Manuela Côrte-Real. “Cathepsin D protects colorectal cancer cells from acetate-induced apoptosis through autophagy-independent degradation of damaged mitochondria”. IMYA 29 setembro-3 outubro, 2015, Porto.

- Sara Alves, Lisandra Castro, Maria Sofia Fernandes, Rita Francisco, Paula Castro, Muriel Priault, Susana Rodrigues Chaves, Mary Pat Moyer, Carla Oliveira, Raquel Seruca, Manuela Côrte-Real, Maria João Sousa, Ana Preto. “Colon cancer-related mutant KRAS alleles function as positive regulators of autophagy”.  IMYA 29 setembro-3 outubro, Porto, 2015

- Oliveira, C.S.F.; Marques, C.; Alves, S.; Castro, L.;  Pereira, H.; Chaves, S.R.; Azevedo-Silva, J.; Casal, M.; Baltazar, F.; Côrte-Real, M.; Preto, A. “Unravel the mechanisms of acetate induced apoptosis in colorectal cancer.” PDBMA Symposium, 26 november 2015, Braga.

2016

- Azevedo-Silva, J, Queirós, O, Ribeiro, A, Baltazar, F, Ko, YH, Petersen, P L, Preto, A, Casal, M. “The extracellular pH influences the cytotoxicity and transport of 3-Bromopyruvate in breast cancer cells.” 2º Congresso Internacional da Associação Portuguesa de Investigação em Cancro (ASPIC), 28-29 abril 2016, Porto.

- Oliveira, CSF, Pereira, H, Alves, S, Castro, L, Baltazar, F, Chaves, SR, Côrte-Real, M and Preto, A. “Cathepsin D-mediated degradation of damaged mitochondria rescues colorectal cancer cells from acetate-induced apoptosis.” 2º Congresso Internacional da Associação Portuguesa de Investigação em Cancro (ASPIC), 28-29 abril 2016, Porto.

- Moreira, T, Sanches, A, Pilon, A, Francisco, R, Ferreira, A, Garcia, MH, Preto, A, Valente, A. “Exploring the effect of multifunctional polymer-ruthenium conjugates: new promising anticancer agentes”. 2º Congresso Internacional da Associação Portuguesa de Investigação em Cancro (ASPIC), 28-29 abril 2016, Porto.

- J. Azevedo-Silva, O. Queirós, A. Ribeiro, F. Baltazar, Y. H. Ko, P. L. Petersen, A. Preto & M. Casal. “The extracellular pH influences the cytotoxicity and transport of 3-Bromopyruvate by monocarboxylate transporters in breast cancer cells.”  XI Jornadas Portuguesas de Genética, 2-3 junho 2016, Universidade de Coimbra, Coimbra.

- A. Matos, F. Marques, R. Francisco, T. Moreira, A. Preto, M. H. Garcia, A. Valente, "Unraveling the mode of action of new promising polymer-ruthenium conjugates”. ULTRAPATH XVIII July 11-15, 2016, Lisbon, Portugal.

- Moreira, T., Francisco, R., Garcia, M.H., Valente, A, Preto, A. “Exploring multifunctional polymer-ruthenium conjugates as new promising anticancer agentes”. Sociedade Portuguesa de Bioquimica (SPB), Guimarães, 8-10 dez 2016.

- Giulia Cazzanelli, Patrícia Carvalho, Carina Cunha, Ana João  Rodrigues, Cândida Lucas, Maria José Oliveira, Ana Preto. “Uncover The Role Of KRAS/Galectin-3/P16ink4a Axis Regulation In Colorectal Cancer”. Sociedade Portuguesa de Bioquimica (SPB), Guimarães, 8-10 dez 2016.

- Anabela Ferreira, Ângela Fazenda, Sara Alves, Manuela Corte-Real, Ana Preto, Maria João Sousa. “Understanding the role of oncogenic KRAS mutations in the regulation of mitochondria and Bax-mediated cell death”. Sociedade Portuguesa de Bioquimica (SPB), Guimarães, 8-10 dez 2016.

 

 

Post-Docs & Students

PhD students (concluded):

- Sara Alves (supervisor, grant FCT SFRH/BD/64695/2009)

- Eugénia Nogueira (supervisor, grant FCT SFRH/BD/81269/2011)

- Suellen Ferro (supervisor, grant FCT SFRH/BD/77449/2011 )

- João Silva (co-supervisor, grant FCT SFRH/BD/76038/2011)

PhD students (ongoing):

- Giullia Cazanelli (co-supervisor, grant Marie Curie - FP7-PEOPLE-2012-ITN proposal n° 317297 – GLYCOPHARM)

- Ricardo Amorim (co-supervisor, grant FCT SFRH/BD/98002/2013)

Master students ongoing (2016/2017):

- Ana Almeida (supervisor) -Master in Molecular Genetics

- Ana Rita Brás (supervisor)-Master in Molecular Genetics

- Flávia Pereira (supervisor)- Master in Molecular Genetics

- Sara Gomes (supervisor)-Master in Molecular Genetics

- Tiago Moreira- (supervisor)-Master in Molecular Genetics

- Anabela Gonçalves- (co-supervisor)-Master in Molecular Genetics

Latest News

You are here: Home People Members

Members' Resources