CBMA

Centro de Biologia Molecular e Ambiental

Centre of Molecular and Environmental Biology

Arunava Pradhan
Arunava Pradhan
...
00351967089602
Post-Doc Researcher
Sustaining Life - Biodiversity and Functional Ecology
Impacts of Emerging chemical contaminants in freshwater ecosystems
CBMA,
Department of Biology
University of Minho - Campus of Gualtar, - 4710-057, Braga - P

Current Research & Key Publications

Nanoparticles are the focus of scientific world in recent years due to their multiple applications for human welfare. Engineered nanoparticles are used in medical, pharmaceutical and therapeutic research, electronics, cosmetics, textiles, antimicrobial paints, ornaments, catalysis, battery and polymer industries, as semiconductors etc. Increasing demands, commercial production, application and usage of metal-based nanoparticles in various fields lead to their accumulation into the environment. Natural water, being the large environmental compartment, is likely to be the terminal repository of these nanoparticles which may pose risk to aquatic biota and associated ecosystem functions. Indeed, many studies with metal-based nanoparticles reported adverse biological effects. This in turn may pose risk to human via food chains / food webs. On the other hand some water soluble carbon-based non metallic nanoparticles have been revealed to have beneficial effects to living organisms. However, the knowledge on impacts to aquatic biota, cellular interactions, biochemical (enzymatic, genetic and proteomic) responses and mechanism of action of the nanoparticles is limited. Moreover, few controversial effects are also reported.

Investigating the physiological, cellular and biochemical responses to metallic nanoparticles (morphological alteration, biosorption and bioaccumulation of nano and/or ionic metals, intracellular reactive oxygen species (ROS) production, plasma membrane integrity, DNA strand breaks, extracellular Laccase production, antioxidant enzymatic activities, activities of neurotoxic biomarkers etc.) in the microbes (in population and community levels) and invertebrates of freshwater benthic detritus food web or those used as laboratory models and the underlying mechanisms / mode of action of nanometal-induced toxicity and role of bioavailable dissolved ionic metals released / leached from nanometals are the major concerns and interests of current research. We are studying the combined and individual biological (microbes and invertebrates) impacts of natural organic matter (NOM) on the toxicity of metal nanoparticles, size dependent toxicity of metal nanoparticles and their interactions on aquatic microbes and invertebrates with mechanistic explanations. Additionally, development in the approach of molecular genomics is ongoing to understand the stress responses of aquatic microbes to nanoparticles.

In contrary, our recent study revealed growth stimulatory and antioxidant impacts of non-metallic carbon-based nanoparticles in yeast with mitigation of ionic metal-induced oxidative stress monitoring the intra- and extracellular interactions (including ligand-binding properties) between nanoparticles and ionic metals which could be potentially implicated in toxicant remediation processes and nanomedicinal and nanotherapeutic fields. We are also investigating the impacts and mode of action of carbon-based nanoparticles as an antioxidant against the nanometal-induced oxidative stress in yeast considering the interactions with macromolecules of media as an approach for the environmental friendly cleaner and biocompatible nanotechnology.

ISI ResearcherID

ORCID Profile

Google Scholar Citations

1. Pradhan A, Seena S, Schlosser D, Gerth K, Helm S, Dobritzsch M, Krauss G-J, Dobritzsch D, Pascoal C, Cássio F. 2015. Fungi from metal-polluted streams may have high ability to cope with the oxidative stress induced by copper oxide nanoparticles. Environmental Toxicology and Chemistry, 34 (4), 923–930 [IF: 3.23; 5-Year IF: 3.25; Q1 in subject category: Environmental Sciences; Q2 in subject category: Toxicology (JCR 2015, Thomson Reuters)]. http://dx.doi.org/10.1002/etc.2879

2. Pradhan A, Geraldes P, Seena S, Pascoal C, Cássio F. 2015. Natural organic matter alters size-dependent effects of nanoCuO on the feeding behaviour of freshwater invertebrate shredders. Science of the Total Environment, 535 (Special Issue: Engineered nanoparticles in soils and waters), 923–930 [IF: 4.1; 5-Year IF: 4.41; Q1 in subject category: Environmental Sciences (JCR 2015, Thomson Reuters)]. http://dx.doi.org/10.1016/j.scitotenv.2014.12.096

3. Pradhan A, Pinheiro JP, Seena S, Pascoal C, Cássio F. 2014. Polyhydroxyfullerene binds cadmium ions and alleviates metal-induced oxidative stress in Saccharomyces cerevisiae. Applied and Environmental Microbiology, 80 (18), 5874–5881 [IF: 3.67; 5-Year IF: 4.36; Q1 in subject categories: Microbiology, Biotechnology and Applied Microbiology (JCR 2015, Thomson Reuters)]. http://dx.doi.org/10.1128/AEM.01329-14

4. Pradhan A, Seena S, Dobritzsch D, Helm S, Gerth K, Dobritzsch M, Krauss G-J, Schlosser D, Pascoal C, Cássio F. 2014. Physiological responses to nanoCuO in fungi from non-polluted and metal-polluted streams. Science of the Total Environment, 466–467, 556–563 [IF: 4.1; 5-Year IF: 4.41; Q1 in subject category: Environmental Sciences (JCR 2015, Thomson Reuters)]. http://dx.doi.org/10.1016/j.scitotenv.2013.07.073

5. Pradhan A, Seena S, Pascoal C, Cássio F. 2012. Copper oxide nanoparticles can induce toxicity to the freshwater shredder Allogamus ligonifer. Chemosphere, 89, 1142–1150 [IF: 3.5; 5-Year IF: 3.87; Q1 in subject category: Environmental Sciences (JCR 2013, Thomson Reuters)]. http://dx.doi.org/10.1016/j.chemosphere.2012.06.001

Research Projects

NANOECOTOX-Impacts of metal nanoparticles to aquatic ecosystems: from community responses to cellular targets, 2012-2015, PTDC/AAC-AMB/121650/2010 at CBMA, Department of Biology, University of Minho, Braga, Portugal – as research grantee (BI2/121650/2013). 184296 €

Effects of silver nanoparticles on litter decomposition and decomposers, FCT-DAAD-2013-2014, Transnational Cooperation – as team member at CBMA, Department of Biology, University of Minho, Braga (Portugal) and Leibniz Institute of Freshwater Ecology and Inland Fisheries-IGB, Berlin, (Germany).

(Micro)analysis of nanoparticles on aquatic fungi, FCT-DAAD-2010-2011, Transnational Cooperation – as team member at CBMA, Department of Biology, University of Minho, Braga (Portugal), Martin Luther University, Halle (Germany) and Helmholtz Centre for Environmental Research - UFZ, Leipzig (Germany).

Publications

1. Pradhan A, Geraldes P, Seena S, Pascoal C, Cássio F. 2016. Humic acid mitigates the toxicity of smaller size copper oxide nanoparticles to microbial decomposers in freshwaters. Freshwater Biology (in press; special issue). [IF: 2.93; 5-Year IF: 3.83; Q1 in Marine and Freshwater Biology (Journal Citation Report 2016, Thomson Reuters)]. http://dx.doi.org/10.1111/fwb.12662

2. Pradhan A, Seena S, Schlosser D, Gerth K, Helm S, Dobritzsch M, Krauss G-J, Dobritzsch D, Pascoal C, Cássio F. 2015. Fungi from metal-polluted streams may have high ability to cope with the oxidative stress induced by copper oxide nanoparticles. Environmental Toxicology and Chemistry, 34 (4), 923–930 [IF: 2.76; 5-Year IF: 3.02; Q2 in Toxicology, Environmental Sciences (JCR 2016, Thomson Reuters)]. http://dx.doi.org/10.1002/etc.2879

3. Pradhan A, Geraldes P, Seena S, Pascoal C, Cássio F. 2015. Natural organic matter alters size-dependent effects of nanoCuO on the feeding behaviour of freshwater invertebrate shredders. Science of the Total Environment, 535 (special issue: Engineered nanoparticles in soils and waters), 94–101 [IF: 3.98; 5-Year IF: 4.32; Q1 in Environmental Sciences (JCR 2016, Thomson Reuters)]. http://dx.doi.org/10.1016/j.scitotenv.2014.12.096

4. Pradhan A, Pinheiro JP, Seena S, Pascoal C, Cássio F. 2014. Polyhydroxyfullerene binds cadmium ions and alleviates metal-induced oxidative stress in Saccharomyces cerevisiae. Applied and Environmental Microbiology, 80 (18), 5874–5881 [IF: 3.67; 5-Year IF: 4.36; Q1 in Microbiology, Biotechnology and Applied Microbiology (JCR 2015, Thomson Reuters)]. http://dx.doi.org/10.1128/AEM.01329-14

5. Pradhan A, Seena S, Dobritzsch D, Helm S, Gerth K, Dobritzsch M, Krauss G-J, Schlosser D, Pascoal C, Cássio F. 2014. Physiological responses to nanoCuO in fungi from non-polluted and metal-polluted streams. Science of the Total Environment, 466–467, 556–563 [IF: 4.1; 5-Year IF: 4.41; Q1 in Environmental Sciences (JCR 2015, Thomson Reuters)]. http://dx.doi.org/10.1016/j.scitotenv.2013.07.073

 6. Pradhan A, Seena S, Pascoal C, Cássio F. 2012. Copper oxide nanoparticles can induce toxicity to the freshwater shredder Allogamus ligonifer. Chemosphere, 89, 1142–1150 [IF: 3.14; 5-Year IF: 3.63; Q1 in Environmental Sciences (JCR 2013, Thomson Reuters)]. http://dx.doi.org/10.1016/j.chemosphere.2012.06.001

 7. Pradhan A, Seena S, Pascoal C, Cássio F. 2011. Can Metal Nanoparticles Be a Threat to Microbial Decomposers of Plant Litter in Streams? Microbial Ecology, 62, 58–68 [IF: 2.91; 5-Year IF: 3.61; Q1 in Marine and Freshwater Biology (Journal Citation Report 2012, Thomson Reuters)]. http://dx.doi.org/10.1007/s00248-011-9861-4

 8. Pradhan A, Bhaumik P, Das S, Mishra M, Khanam S, Hoque BA, Mukherjee I, Thakur AR, Ray Chaudhuri S. 2008. Phytoplankton Diversity as Indicator of Water Quality for Fish Cultivation. American Journal of Environmental Sciences, 4, 406–411 [IF: 0.42 (SCImago Journal Rank 2008)]. http://dx.doi.org/10.3844/ajessp.2008.406.411

 

 

 

 

GenBank Submission(s):

16S Ribosomal RNA gene, partial sequence of 25 novel bacterial clones (Accession numbers: EF536721-EF536745). Authors: Raychaudhuri S, Pradhan A, Bhaumik P, Chakraborty S, April 2, 2007.

Doctoral Thesis in Science (with specialization in Biology):

Impacts of nanoparticles to microbes and invertebrates: from community responses to cellular targets. Defended on September 6, 2013, School of Science, University of Minho,  Braga, Portugal.

A. Oral Communication(s):

 

1. Pradhan A, Silva C, Silva C, Pascoal C and Cássio F. 2015. “Biomarker responses to nanoCuO-induced oxidative and neuronal stress in freshwater caddisfly larvae”. 9th Symposium for European Freshwater Sciences (SEFS9), July 5-10, Geneva, Switzerland (accepted).

2. Tlili A1, Pradhan A1, Pascoal C, Cássio F, Gessner MO. 2015. Responses of stream microbial communities to nanosilver: search for effective stress indicators”. 25th SETAC Europe Annual Meeting, May 3-7, Barcelona, Spain. 1Equal authorship.

3. Carvalho F, Pradhan A, Abrantes N, Pascoal C and Cássio F. 2015. Ecotoxicological effects of post-fire runoffs on aquatic invertebrate detritivores”. 25th SETAC Europe Annual Meeting, May 3-7, Barcelona, Spain.

4. Pradhan A, Pascoal C and Cássio F. 2014. “Engineered nanomaterials in the environment: cellular to ecological impacts”. Invited speaker, International Workshop on Nanoparticles for soil remediation: from green synthesis to ecotoxicological effects, Escola Superior Agrária de Coimbra, December 19, Coimbra, Portugal.

5. Cássio F, Pradhan A, Pascoal C. 2014. Environmental impacts of nanoparticles: from cells to ecosystems”. International workshop on Nanotechnology applied to environmental Geotechnics (December 4-5), organized by University of Minho, Portuguese Environmental Geotechnics Commission and Portuguese Geotechnical Society, December 4, Braga, Portugal.

6. Pradhan A. 2014. “Impacts of nanoparticles to microbes and invertebrates: from community responses to cellular targets”. Invited speaker, Biotechnology Alumni Seminar Series, 2014, October 27, West Bengal University of Technology, Kolkata, India.

7. Pradhan A, Seena S, Pascoal C and Cássio F. 2013. O poli-hidroxi-fulereno pode estimular o crescimento de leveduras e mitigar o stress oxidativo induzido pelo cádmio”. Encontro Nacional de nanotoxicologia (E2N, April 2-3), April 2, Lisbon, Portugal.

8. Pradhan A, Geraldes P, Seena S, Pascoal C and Cássio F. 2012. Humic acids and nanoparticle size change the toxicity of nano CuO to freshwater microbes and invertebrates”. Limnologia 2012 (July 2-6), 16th International Congress of the Iberian Association of Limnology, July 6, University of Minho, Guimarães, Portugal.

9. Pradhan A, Seena S, Helm S, Gerth K, Schlosser D, Krauss G, Wesenberg D, Pascoal C and Cássio F. 2012. “Responses of freshwater microbial decomposers to copper oxide nanoparticles. Limnologia 2012 (July 2-6), 16th International Congress of the Iberian Association of Limnology, University of Minho, July 6, Guimarães, Portugal.

10. Pradhan A, Seena S, Pascoal C and Cássio F. 2012. “Toxicity of nano CuO in aquatic food webs: from microbes to invertebrate”. DBio Seminars, CBMA, Department of Biology, University of Minho, March 21, Braga, Portugal.

11. Pradhan A, Seena S, Pascoal C and Cássio F. 2010. “Impacts of metal nanoparticles on leaf litter decomposition by aquatic microbes”. DBio Seminars, CBMA, Department of Biology, University of Minho, May 26, Braga, Portugal.

12. Pradhan A, Seena S, Pascoal C and Cássio F. 2009. “Impacts of metal nanoparticles on leaf litter decomposition by aquatic fungi”. BioMicroWorld (December 2-4), 3rd International Conference on Environmental, Industrial and Applied Microbiology, University of Lisbon, December 2, Lisbon, Portugal.

B. Poster Communication(s):

1. Pradhan A. 2015. Impacts of nanoparticles to microbes and invertebrates: from community responses to cellular targets”. 9th Symposium for European Freshwater Sciences (SEFS9), July 5-10, Geneva, Switzerland. Invited for poster presentation on PhD work by association of European Fresh and Young Researchers (EFYR) as winner of EFFS subsidiary Award 2015 for Best PhD Dissertation (2013-2014) in Freshwater Sciences.

2. Barros D, Pradhan A, Santos PM, Pascoal C and Cássio F. 2015. Responses of antioxidant enzymes to nanoparticulate and ionic silver in aquatic microbes”. 25th SETAC Europe Annual Meeting, May 3-7, Barcelona, Spain.

3. Pradhan A1, Silva C1, Pascoal C and Cássio F. 2014. “Neurotoxicity and oxidative stress induced by copper oxide nanoparticles and ionic copper in freshwater invertebrate shredders”. Limnologia, 18th International Congress of the Iberian Association of Limnology, July 6-11, University of Cantabria, Santander, Spain. 1Equal authorship.

4. Pradhan A, Geraldes P, Seena S, Pascoal C and Cássio F. 2013. “Sublethal toxicity of nanoCuO to freshwater shredders can be influenced by nanoparticle size and humic acids”. 23rd SETAC Europe Annual Meeting, May 12-16, Glasgow, UK.

5. Pradhan A, Geraldes P, Seena S, Pascoal C and Cássio F. 2013. “NanoCuO toxicity to freshwater microbial decomposers depends on nanoparticle size and humic acids”. 23rd SETAC Europe Annual Meeting, May 12-16, Glasgow, UK.

6. Pradhan A, Geraldes P, Seena S, Pascoal C, Cássio F. 2013. A toxicidade das nanopartículas de óxido de cobre para os microrganismos aquáticos decompositores depende do tamanho das nanopartículas e da presença de ácidos húmicos”. Encontro Nacional de nanotoxicologia (E2N), April 2-3, Lisbon, Portugal.

7. Pradhan A, Geraldes P, Seena S, Pascoal C, Cássio F. 2013. “Efeito do tamanho das nanopartículas de óxido de cobre e da presença de ácidos húmicos no invertebrado detritívoro Allogamus ligonifer”. Encontro Nacional de nanotoxicologia (E2N), April 2-3, Lisbon, Portugal.

8. Pradhan A, Seena S, Helm S, Gerth K, Krauss GJ, Wesenberg D, Schlosser D, Pascoal C and Cássio F. 2012. Effects of nano CuO on aquatic decomposers: from community to cellular responses”. 22nd SETAC Europe Annual Meeting and 6th SETAC World congress, May 20-24, Berlin, Germany (invited for poster corner).

9. Pradhan A, Seena S, Pascoal C and Cássio F. 2012. “Polyhydroxyl fullerene can mitigate toxicity effects of cadmium on yeasts”. 22nd SETAC Europe Annual Meeting and 6th SETAC World congress, May 20-24, Berlin, Germany.

10. Pradhan A, Seena S, Pascoal C and Cássio F. 2011. “Impacts of CuO nanoparticles on aquatic detritus foodwebs”. 6th International Conference on the Environmental Effects of Nanoparticles and Nanomaterials, the Royal Society, September 19–21, London, United Kingdom.

11. Pradhan A, Seena S, Pascoal C and Cássio F. 2011. “Nano copper oxide is a threat to an endemic shredder of the Iberian Peninsula”. 21st SETAC Europe Annual Meeting, May 15-19, Milan, Italy.

12. Pradhan A, Schlosser D, Seena S, Helm S, Gerth K, Krauss GJ, Wesenberg D, Pascoal C and Cássio F. 2011. “Copper oxide nanoparticles induce oxidative stress, DNA strand breaks and laccase activity in aquatic fungi”. 21st SETAC Europe Annual Meeting, May 15-19, Milan, Italy.

13. Pradhan A, Seena S, Pascoal C and Cássio F. 2010. “Nano metals may pose negative impacts to microbial decomposition in streams”. 20th SETAC Europe Annual Meeting, May 23-27, Seville, Spain.

14. Mishra M, Nasipuri P, Pradhan A, Thakur AR and Raychaudhuri S. 2007. “Sulfate Reducing Bacteria – A Promising Candidate for Environmental Sulfate Removal” in International Congress of Environmental Research (ICER 2007), December 28-30, Bhopal, Madhya Pradesh, India.

15. Pradhan A. 2006. “Nurture Nature for the Future”. National Science Day (DBT sponsored), West Bengal University of Technology, February 28, Kolkata, India.

Post-Docs & Students

 

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