CBMA

Centro de Biologia Molecular e Ambiental

Centre of Molecular and Environmental Biology

Odília dos Anjos Pimenta Marques de Queirós
Odília dos Anjos Pimenta Marques de Queirós
...
00351224157126
Assistant Professor/Researcher
Basics of Life - Cellular Responses to Environmental Stress
Toxicology and Metabolism
Instituto Superior de Ciências da Saúde-Norte, R. Central de Gandra, 1317,

4585-116 Gandra-P.

Universidade do Minho - Campus de Gualtar, Departamento de Biologia - 4710-057, Braga - P

Current Research & Key Publications

Odília Queirós holds a Doctoral Degree in Biological Sciences at the Sciences School of the University of Minho, a Master in Molecular Genetics at the same University and a Degree in Biochemistry at the Faculty of Sciences and Abel Salazar Biomedical Sciences Institute of the University of Oporto. Presently, she is Assistant Professor at the Advanced Institute of Health Sciences – North (ISCSN/CESPU), where she lectures curricular units in the field of Biochemistry and Molecular Biology. She is the Coordinator of the BSc in Nutrition Sciences and member of the Coordination Committee of the Master in Molecular Therapies at the same Institution. She is a researcher integrated in CBMA. Her research is focused in the study of cancer cell metabolism and in the antitumor activity of drugs acting on metabolic targets as well as on the study of molecular mechanisms associated with multidrug resistance (MDR) phenotype in cancer cells. 

Tumor metabolism and microenvironment are pivotal factors in tumorigenesis, influencing tumor growth, angiogenesis, invasion and resistance to treatment. Cancer cells produce ATP mainly by glycolysis, even in normoxia, a phenomenon known as “Warburg effect”. The synthesis of novel antitumor compounds targeting cell metabolism increased in the last years, aiming at a more specific and efficient cancer treatment. Our research is focused in the study of cancer cell metabolism and in the importance of membrane transporters in the antitumor activity of drugs acting on metabolic targets as well as the role of the carboxylic acids in this process. We aim also to study the molecular mechanisms associated with multidrug resistance (MDR) phenotype in cancer cells, namely concerning the activity of ABC transporters and the role of the cancer cell metabolism, of the expression of pH regulators and of the tumor microenvironment in this phenotype.The assessment of the role of MDR genes polymorphisms in the reponse to antitumor agents, using models of simple eukaryotes, such as yeast, for the heterologous  expression of different variants of the genesis, is also a research goal. 

 

Tavares-Valente D, Baltazar F, Moreira R, Queirós O. (2013) Cancer cell bioenergetics and pH regulation influence breast cancer cell resistance to paclitaxel and doxorubicin. J Bioenerg Biomembr. 45: 467-475. doi: 10.1007/s10863-013-9519-7

Queirós, O., Preto, A., Pacheco, A., Pinheiro, C., Azevedo-Silva, J., Moreira, R., Pedro, M., Ko, Y.H., Pedersen, P., Baltazar, F. and Casal, M. (2012) Butyrate activates the monocarboxylate transporter MCT4 expression in breast cancer cells and enhances the antitumor activity of 3-bromopyruvate. J Bioenerg Biomembr. 44: 141-153. doi: 10.1007/s10863-012-9418-3.

Pacheco, A., Talaia, G., Sá-Pessoa, J., Bessa, D., Gonçalves, MJ., Moreira, R., Paiva, S., Casal, M. and Queirós, O. (2012) Lactic acid production in Saccharomyces cerevisiae is modulated by the expression of Jen1 and Ady2. FEMS Yeast research. 12: 375-381. doi: 10.1111/j.1567-1364.2012.00790.x.

Casal M., Paiva, S. Queirós, O. and Silva, I.S. (2008). Transport of carboxylic acids in yeasts. FEMS Microbiol Reviews. 32:974-994. doi: 10.1111/j.1574-6976.2008.00128.x.

Research Projects

 “MycoFat: Metabolic engineering of yeast fatty acids synthesis for biodiesel production”. FCT. PTDC/AAC-AMB/120940/2010. Member of the team

“Using waste glycerol biodiesel derived for the synthesis of high-value compounds”. Funding: CESPU. PROJECT Nº 02-GBMC-CICS-11 Institution: Instituto Superior de Ciências da Saúde, Norte.Principal Investigator

“Populational studies in MDR1 genetic polymorphisms”. Funding: CESPU. PROJECT Nº 03-GBMC-CICS-11 Institution: Instituto Superior de Ciências da Saúde, Norte.Member of the team

 

Publications

Since 2008

Tavares-Valente D, Baltazar F, Moreira R, Queirós O. (2013) Cancer cell bioenergetics and pH regulation influence breast cancer cell resistance to paclitaxel and doxorubicin. J Bioenerg Biomembr. 45: 467-475. doi: 10.1007/s10863-013-9519-7

Barbosa J, Faria J, Carvalho F, Pedro M, Queirós O, Moreira R, Dinis-Oliveira RJ. (2013) Hair as an alternative matrix in bioanalysis. Bioanalysis. 5:895-914. doi: 10.4155/bio.13.50.

Queirós, O., Preto, A., Pacheco, A., Pinheiro, C., Azevedo-Silva, J., Moreira, R., Pedro, M., Ko, Y.H., Pedersen, P., Baltazar, F. and Casal, M. (2012) Butyrate activates the monocarboxylate transporter MCT4 expression in breast cancer cells and enhances the antitumor activity of 3-bromopyruvate. J Bioenerg Biomembr. 44: 141-153. doi: 10.1007/s10863-012-9418-3.

Pacheco, A., Talaia, G., Sá-Pessoa, J., Bessa, D., Gonçalves, MJ., Moreira, R., Paiva, S., Casal, M. and Queirós, O. (2012) Lactic acid production in Saccharomyces cerevisiae is modulated by the expression of Jen1 and Ady2. FEMS Yeast research. 12: 375-381. doi: 10.1111/j.1567-1364.2012.00790.x.

Casal M., Paiva, S. Queirós, O. and Silva, I.S. (2008). Transport of carboxylic acids in yeasts. FEMS Microbiol Reviews. 32:974-994. doi: 10.1111/j.1574-6976.2008.00128.x.

 Since 2008

Tavares Valente D, Baltazar F, Moreira R and Queirós O (2014). Exploiting cell metabolism in cancer therapy: Effect of bioenergetic modulators on tumor cell characteristics. Front. Pharmacol. Conference Abstract: 4th Annual Meeting of the International Society of Proton Dynamics in Cancer. doi: 10.3389/conf.fphar.2014.61.00046

Azevedo-Silva J, Queirós O, Ko YH, Pedersen P, Preto A and Casal M(2014). Characterization of 3-bromopyruvate uptake in breast cancer cells. Front. Pharmacol. Conference Abstract: 4th Annual Meeting of the International Society of Proton Dynamics in Cancer. doi:10.3389/conf.fphar.2014.61.00003

 D. Valente, A. Cunha, C. Pinheiro, F. Baltazar, R. Moreira and O. Queirós. Effect of efflux pumps expression and cell bioenergetics in the multidrug resistance phenotype in cancer cells. Exp Clin Endocrinol Diabetes 2012; 120 - A35. Metabolism 2012, From signalling to disease. Novembro 2012, Heidelberg, Germay. DOI: 10.1055/s-0032-1330830.

 A. Pacheco, O. Queirós, A. Preto, C. Pinheiro, J. Azevedo-Silva, R. Moreira, M. Pedro, F. Baltazar and M. Casal 3-bromopyruvate cytotoxic effect in breast cancer cells is dependent of monocarboxylate transporters (MCT) expression and is enhanced by butyrate. Exp Clin Endocrinol Diabetes 2012; 120 - A22. Metabolism 2012, From signalling to disease. Novembro 2012, Heidelberg, Germany. DOI: 10.1055/s-0032-1330817.

 E. Silva, M. Casal, S. Paiva and O. Queirós. Subcellular localization of the monocarboxylate transporter Mct1 tagged with the green fluorescence protein in different cancer cell lines. Exp Clin Endocrinol Diabetes 2012; 120 – A30. Metabolism 2012, From signalling to disease. Novembro 2012, Heidelberg, Germany. DOI: 10.1055/s-0032-1330825.


 

 

 

 Oral communications by invitation:

O. Queirós. Cell metabolism as target for cancer therapy. Seminários do Departamento de Biologia da Universidade do Minho no âmbito da comemoração dos 20 anos do Mestrado em Genética Molecular. May 2013.

O. Queirós. Novos Mercados de Trabalho. I Jornadas de Empreendedorismo e Novos Mercados de Trabalho em Nutrição (ANEN). Novembro 2012. Palestra oral por convite e participação em mesa redonda.

Queirós, O, Falcão-Moreira R. “Microfábricas de Sucesso”. XVII Congresso nacional de Bioquímica.  Porto, December 2010.

Oral Communications: 

J. Azevedo-Silva, O. Queirós, Y.H. Ko, P.Pedersen, A. Preto and M. Casal. Characterization of 3-bromopyruvate uptake in breast cancer cells. 4th International Society of Proton Dynamics in Cancer. Munich, Germany. October 2013.

M. Casal, A. Pacheco, J. Sá-Pessoa, A. Preto, J. Azevedo-Silva, C. Pinheiro, F. Baltazar, Y. H. Ko, P. L. Pedersen, S. Paiva and Odília Queirós. Transport of lactic acid across plasma membrane: implications in health and biotechnology. SMYTE30. Salamanca, Julho de 2012.

A. Pacheco, D. Bessa, MJ Gonçalves, R. Moreira, M. Casal and O. Queirós. “Enhancement of carboxylic acids production in yeast: the role of carboxylic acids permeases”, International Yeast Meeting Convention 2010 Tribute to P. Slonimski, Roma Setembro de 2010.

D. Bessa, A. Pacheco, E. Almeida, R. Moreira, I. Soares-Silva, M. Casal and O. Queirós . Metabolic engineering of Saccharomyces cerevisiae for lactic and citric acids production from raw glycerol. XVII Jornadas de Biologia de Leveduras “Nicolau van Uden”. Universidade do Minho, Braga. 2009

T. Silva, D. Santos, M.J. Barbosa, J. Faria, H. Bousbaa, O. Queirós, P. Moradas-Ferreira, A.M. Damas,F. Ferreira-da-Silva and R. Falcão-Moreira. Regulation of expression and subcellular localization of kmGAPDH1p in Kluyveromyces marxianus. XVII Jornadas de Biologia de Leveduras “Nicolau van Uden”. Universidade do Minho, Braga. 2009

Posters:

Queirós, O., Bousbaa, H., Cardoso, E. and Moreira, R. Tutorial project in the Biochemistry degree of ISCS-N: A valuable approach in biochemical education. XVI Congresso Nacional de Bioquímica, November 2008. Azores

J. Faria, A.V. Nascimento, M.J. Barbosa, R.F. Moreira, O. Queirós and H. Bousbaa, Inquiring the competence of the mitotic checkpoint in an oral squamous cell carcinoma line. XVI Congresso Nacional de Bioquímica, November 2008. Azores

M.J. Barbosa, A.V. Nascimento, J. Faria, O. Queirós, R.F. Moreira and H. Bousbaa, Study of the mitotic checkpoint competence in a medulloblastoma cell line. XVI Congresso Nacional de Bioquímica, November 2008. Azores

A. Pacheco, D. Bessa, E. Almeida, R. Moreira, I. Soares-Silva, M. Casal and O. Queirós. Metabolic engineering of Saccharomyces cerevisiae towards lactic and citric acids production from raw glycerol. III Jornadas de Ciências do ISCSN. 2009. Porto

Faria JF, Barbosa MJ, Nascimento AV, Falcão-Moreira R, Queirós O, Bousbaa H. Altered expression of the mitotic checkpoint genes in an oral squamous cell carcinoma cell line. III Jornadas de Ciências do ISCSN. 2009. Porto

Silva T, Santos D, Monteiro I, Blanquett R , Barbosa MJ, Faria JF, Bousbaa H, Queirós O, Moradas-Ferreira P, Ferreira-da-Silva F, Falcão-Moreira R. Endereçamento não clássico e regulação da expressão da GAPDH1p (p37) de Kluyveromyces marxianus - estratégias de estudo. III Jornadas de Ciências do ISCSN. 2009. Porto 

Beatriz Barata, Zozefina Foskolou, Daniela Bessa, António Pacheco, Odília Queirós, Margarida Casal and Isabel Soares-Silva. Study of Jen1p homologs by heterologous expression in Saccharomyces cerevisiae. National Congress MicroBiotec09. 2009. Vilamoura.

Barbosa MJ, Faria JF, Silva T, Bousbaa H, Queirós O, Moradas-Ferreira P, Ferreira-da-Silva F, Falcão-Moreira R. Non-classical targeting and regulation of expression of GAPDH1 from Kluyveromyces marxianus. National Congress MicroBiotec09. 2009. Vilamoura. 

João Lopes, Daniela Bessa, Sandra Paiva, Margarida Casal, Bjorn Johansson and Odília Queirós. Metabolic engineering of a Saccharomyces cerevisiae strain improved its citric acid production. XXXV Jornadas de Genética. 2010. Braga.

A. Pacheco, D. Bessa, T. Mendes, MJ Gonçalves, R. Moreira, M. Casal and O. Queirós. Lactic acid production in yeast is modulated by the expression of carboxylic acids permeases. XVII Congresso Nacional de Bioquímica. 2010. Porto.

Faria JF, Barbosa MJ, , Silva T, Bousbaa H, Queirós O, Moradas-Ferreira P, Ferreira-da-Silva F, Bousbaa H, Queirós O, Falcão-Moreira R. Non-classical targeting and regulation of the expression of GAPDH1p from K. marxianus. XVII Congresso Nacional de Bioquímica. 2010. Porto.

Rodrigues G., Ribeiro A., Costa B., Dias M., Ferreira M., Morais R., Azevedo R., Leão A., Queirós O., Moreira R. Projecto Nuteduc – estudo de hábitos alimentares de crianças do 3º e 4º anos das escolas de 1º  ciclo do concelho de Paredes. IX Congresso de Nutrição e Alimentação. 2010. Lisboa

Daniela Bessa, Sandra Paiva, Roxana Moreira, Margarida Casal and Odília Queirós. Functional characterization of Yarrowia lipolytica gene family coding for carboxylic acids permeases homologues. Small Meeting on Yeast Transport and Energetics, SMYTE, Setembro 2011, Mérida, México. 

A. Pacheco, O. Queirós, A. Preto, C. Pinheiro, J. Azevedo-Silva, R. Moreira, M. Pedro, F. Baltazar and M. Casal. Butyric acid increases monocarboxylate transporters (MCT) expression and enhances the cytotoxicity of the anti-tumoral agent 3-bromopyruvate (3-BP) in breast cancer cells. XXI Porto Cancer Meeting. Metabolism and cancer, from etipathogenesis to therapy .Abril 2012, Porto.

D. Valente, C. Pinheiro, F. Baltazar, M. Casal, R. Moreira and O. Queirós. Molecular mechanisms underlying multidrug resistance in cancer cells: targeting cell metabolism and pH regulation. XXI Porto Cancer Meeting. Metabolism and cancer, from etipathogenesis to therapy .Abril 2012, Porto.

A. Cunha, J. Barbosa, J. Faria, M. Pedro, R. Jorge Dinis-Oliveira, O. Queirós and R. Moreira. Genotype frequencies of CYP2D6*4 and CYP2C19*17 single nucleotide polymorphisms in a Portuguese Population. XXXVII Jornadas Portuguesas de Genética. Junho 2012. Lisboa. 

D. Valente, A. Cunha, C. Pinheiro, F. Baltazar, R. Moreira and O. Queirós. Effect of efflux pumps expression and cell bioenergetics in the multidrug resistance phenotype in cancer cells. Metabolism 2012, From signalling to disease, Novembro 2012, Heidelberg, Alemanha.

A. Pacheco, O. Queirós, A. Preto, C. Pinheiro, J. Azevedo-Silva, R. Moreira, M. Pedro, F. Baltazar and M. Casal. 3-bromopyruvate cytotoxic effect in breast cancer cells is dependent of monocarboxylate transporters (MCT) expression and is enhanced by butyrate. Metabolism 2012, From signalling to disease, Novembro 2012, Heidelberg, Alemanha.

E. Silva, M. Casal, S. Paiva and O. Queirós. Subcellular localization of the monocarboxylate transporter Mct1 tagged with the green fluorescence protein in different cancer cell lines. Metabolism 2012, From signalling to disease, Novembro 2012, Heidelberg, Alemanha.

D. Valente, F. Baltazar, R. Moreira and O. Queirós. Multidrug resistance phenotype: pH regulation in breast cancer cells. EMBO YSF. Julho 2013, Lisboa, Portugal.

E. Silva, M. Casal, S. Paiva and O. Queirós. Intracellular trafficking of MCT1-tagged GFP. EMBO YSF. Julho 2013, Lisboa, Portugal.

A. Cunha. O. Queirós and R. Moreira. Population Analysis and Functional Genetic Polymorphisms of CYP2D6*4 and CYP2C19*17 Cytochrome P450 and C3435T MDR1 Gene. ESPT 2013. Second conference: Pharmacogenomics: From cell to clinic. Setembro 2103. Lisboa, Portugal.

D. Valente, F. Baltazar, R. Moreira and O. Queirós. Exploiting cell metabolism in cancer therapy: Effect of bioenergetic modulators on tumor cell characteristics. 4th International Society of Proton Dynamics in Cancer . Munique, Outubro 2013. 

Post-Docs & Students

PhD Students 

“A comparative study of the toxicological effects and pharmacodynamics of tramadol and tapentadol: clinical and forensic implications ". Juliana da Conceição Fernandes de Faria. PhD in Biomedicine. Faculdade de Medicina da Universidade do Porto. Since 2012. Co-Supervisor

“The influence of cancer cell metabolism and microenvironment on tumor progression and drug resistance". Diana Maria Tavares Valente. PhD in Health Sciences. Escola de Ciências da Saúde da Universidade do Minho. Since 2013.

Master students

“Molecular mechanisms underlying multidrug resistance phenotype in cancer cells: targeting cellular bioenergetics and pH regulation". Diana Maria Tavares Valente. Master in Molecular Therapies. Instituto Superior de Ciências da Saúde-Norte. 2013.

“Endocytosis and Intracellular Trafficking of Plasma Membrane Transporters in Mammalian Cells ". Elsa Daniela Gonçalves Silva. Master in Molecular Genetics. Escola de Ciências da Universidade do Minho. 2013.

“Análise Populacional e Funcional dos Polimorfismos Genéticos CYP2D6*4 e CYP2C19*17 do Citocromo P450 e C3435T do Gene MDR1". Andrea Teixeira da Cunha. Master in Molecular Therapies. Instituto Superior de Ciências da Saúde-Norte. 2013. Co-Supervisor

"Avaliação da atividade antitumoral do 3-bromopiruvato. Papel dos transportadores de monocarboxilatos (MCTs) no mecanismo de acção". António Augusto Vieira Pacheco. Master in Molecular Therapies. Instituto Superior de Ciências da Saúde-Norte. 2012.

“Metabolic engineering of the yeast Saccharomyces cerevisiae for citric acid and fatty acids production". João Miguel Pinheiro Lopes. Master in Molecular Genetics. Escola de Ciências da Universidade do Minho. 2012

“Estudos fisiológicos e moleculares de homólogos de transportadores de ácidos carboxílicos em Yarrowia lipolytica". Daniela Sofia dos Santos Bessa. Master in Molecular Genetics. Escola de Ciências da Universidade do Minho. 2011.

“Análise do checkpoint mitótico em células tumorais". Co-orientação de Juliana da Conceição Fernandes de Faria. Mster in Biochemistry. Faculdade de Ciências da Universidade do Porto. 2008.

“Unravelling the molecular mechanisms underlying 3-bromopyruvate resistance in tumor cell lines". Ana Margarida Oliveira Barbosa. Master in Molecular Therapies. Instituto Superior de Ciências da Saúde-Norte. Since 2013.

“Influence of MCT1 and MCT4 polymorphisms on 3-bromopyruvate cytotoxic effect on cancer cells". Joana Catarina Vieira Miranda. Master in Molecular Therapies. Instituto Superior de Ciências da Saúde-Norte. Since 2013.

 

 

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