CBMA

Centro de Biologia Molecular e Ambiental

Centre of Molecular and Environmental Biology

Margarida Casal
Margarida Casal
...
253604310
Full Professor
Innovation for Life - Functional Genomics and Nanobiotechnology for Sustainable Living
Molecular Biotechnology
Departamento de Biologia, Campus de Gualtar, Braga

Profile

MSc, PhD, Habilitation in Biology

Current Research & Key Publications

Main scientific interests and expertise

Biology, Microbiology, Microbial Molecular Genetics, Yeast Classic and Molecular Genetics. Molecular Biotechnology. Structural Biology. Synthetic Protein Biotechnology.

My academic profile can be checked in:

http://scholar.google.pt/citations?user=02W9yO4GjtsC&

http://www.researcherid.com/rid/B-6386-2009

http://orcid.org/0000-0002-0143-9758

Scopus Author ID 7102313058.

I published 9 book chapters, 9 patents and 83 full articles (first one published in 1995) that accumulate at the present time 2322 citations (ISI), which corresponds to an h-index of 27 (February, 2017).

I was invited as a speaker to 45 lectures in national and international symposia. I am Referee of 21 Journals indexed in ISI and Scopus. I have evaluated research projects submitted to funding agencies from Austria, Czech Republic and Belgium as well as from FCT, FP7 and IFS.

I was the Head of the Centre of Molecular and Environmental Biology awarded by FCT with EXCELLENT in the 2013 Evaluation of R&D Units

I am the Coordinator of the FCT Doctoral Program in Applied and Environmental Microbiology (DP_AEM).

 

My current scientific interests are in:

i) Structural-functional analysis of plasma membrane transporters. The main outcomes of my work is the uncover of the genes encoding monocarboxylic acid transporters Jen1 and Ady2 in yeast, and YaaH/SatP in bacteria. By using an assortment of biological materials, covering bacteria, yeasts and human cancer cells, I am focused on solving the structure of carboxylic acid permeases and understand how they work and selectively transport the carboxylic acids.

ii) Recombinant protein-based polymers (rPBPs). The aim is of my research is to design, produce, characterize and develop applications for rPBPs inspired on the structure of silk and elastin, providing an extraordinary tool to create self-structured and “smart” (stimuli responsive) biopolymers. The use of rPBPs inspired on elastin and silk strutures is being oriented for biotechnology and biomedical applications, such as tissue engineering scaffolds. In the scope of this developments in 2012 I was founder and currently partner of the spin-off company NanoDelivery, I&D in Bionanotechnology, Lda.

 

Present positions

- Full Professor - Professora Catedrática, School of Sciences, University of Minho

- Dean of the School of Sciences, University of Minho, 2016 – present

- Director of the Doctoral Program in Applied and Environmental Microbiology (DP_AEM) 

 Vice-President of the Portuguese Genetics Society, 2010-present (elected)

- Member of the Fiscal Council of the Portuguese Biochemistry Society, 2016-present (elected).

 Last 3 years:

A-72. da Costa, A., Machado, R., Ribeiro, A., Collins, T., Thiagarajan, V., Neves-Petersen, M.T., Rodríguez-Cabello, J.C., Gomes A.C., Casal, M.. 2015. Development of Elastin Like Recombinamer films with antimicrobial activity. Biomacromolecules 15, 625?635.

A-73. João Azevedo-Silva, Odília Queirós, Ana Ribeiro, Fátima Baltazar, Ko H Young, Peter L Pedersen, Ana Preto and Margarida Casal. 2015. The cytotoxicity of 3-Bromopyruvate in breast cancer cells depends on extracellular pH. Biochem. J. 467, 247–258.

A-74. Sá-Pessoa, J., Amillis, S., Casal *, M., Diallinas, G. * corresponding author. 2015. Expression and specificity profile of the major acetate transporter AcpA in Aspergillus nidulans. Fungal Genetics and Biology 76: 93–103.

A-75. Machado, R. da Costa, A., Sencadas, V., Pereira, M., Collins, T., Rodríguez-Cabello, J.C., Lanceros-Méndez, S., Casal, M. 2015. Exploring the properties of genetically engineered silk-elastin-like protein films Macromolecular Bioscience 15, 1698–1709

A-76. Mota S., Alves R., Carneiro C., Silva S., Brown A.J., Istel F., Kuchler K., Sampaio P., Casal M., Henriques M., Paiva S. 2015. Candida glabrata susceptibility to antifungals and phagocytosis is modulated by acetate. Frontiers in Microbiology. Vol 6. Article 919. doi: 10.3389/fmicb.2015.00919, 2015M

A-77. Padrão, J., Machado, R., Casal, M., Lanceros-Méndez, S., Rodrigues, L. R. Dourado, F., Sencadas, V. 2015. Antibacterial performance of bovine lactoferrin-fish gelatine electrospun membranes. International Journal of Biological Macromolecules 81: 608-614.

A-78. Soares-Silva I, Ribas D, Foskolou IP, Barata B, Bessa D, Paiva S, Queirós O, Casal M. 2015. The Debaryomyces hansenii carboxylate transporters Jen1 homologues are functional in Saccharomyces cerevisiae. FEMS Yeast Res. 15.

A-79. Azevedo-Silva J, Queirós O, Baltazar F, Ulaszewski S, Goffeau A, Ko Y H, Pedersen P L, Preto A, Casal M. 2016. The Anticancer Agent 3-Bromopyruvate: A Simple But Powerful Molecule Taken From The Lab To The Bedside. J Bioenerg Biomembr. epub ahead of print. doi: 10.1007/s10863-016-9670-z

A-80. Ferro, S., Azevedo-Silva, J., Casal, M., Côrte-Real, M., Baltazar, F., Preto, A. (2016). Characterization of acetate transport in colorectal cancer cells and potential therapeutic implications Oncotarget 7 (43), 70639-70653

A-81. Barroca, M., Rodrigues, P., Sobral, R., Costa, M.M., Chaves, S., Machado, R., Casal, M., Collins, T. 2016. Antibiotic free selection for the high level biosynthesis of a silkelastin-like protein. Scientific Reports. DOI: 10.1038/srep39329.

A-82. Pereira, A.M., Machado, R., da Costa, A., Ribeiro, Collins, T., Gomes, A. C., Leonor, I., Kaplan, D. L., Reis, R.L., Casal, M. (2016, on line) Silk-based biomaterials functionalized with fibronectin type II promotes cell adhesion. Acta Biomaterialia, 47, 1 January 2017, Pages 50–59

A-83. Ribas,D., Sa-Pessoa, J., Soares-Silva, I., Paiva, S., Nygard, Y., Ruohonen, L., Penttila?, M., Casal, M. 2017. Yeast as a tool to express sugar acid transporters with biotechnological interest FEMS Yeast Research 2017;

           doi: 10.1093/femsyr/fox005

Research Projects

IMG 1702--corte-- siteTRANSPROT - New insights into structure, function and regulation of transport proteins. ERASMUS Intensive programme. Nº 2013-PT1-ERA10-16664. PI.

 On-going

EcoAgriFood: Innovative green products and processes to promote Agri-Food BioEconomy,  NORTE-01-0145-FEDER-000009. 2016- 2019. Funding:  1.289.747,00€ (PI)

TransAcids - Estudos estrutura-função de transportadores de ácidos carboxílicos: novas ferramentas na otimização da produção de estirpes microbianas industriais. PTDC/BIA-MIC/5184/2014. Funding: €199.620,00 (PI)

Fun Bio Plas ERA-IB-2-6/0004/2014. Novel synthetic biocomposites for biomedical devices. Funding: €200.000,00. (team member). 

PRINCIPAL INVESTIGATOR:


FP7-NMP-2009-SME-3. Collaborative Project. ECOplast—Research in new biomass-based composites from renewable resources with improved properties for vehicle parts moulding. Workpackage leader.

POCTI/ 1999/ BME / 36625 (PI) - Molecular and genetic analysis of monocarboxylic acid transporters in yeasts

POCTI/BIO/38106/2001 (PI) - Engineering of Saccharomyces cerevisiae species able to remove malic acid under genetic control. 

POCI/CTM/57177/2004 (PI) - Polímeros elastoméricos proteicos: da síntese à moldação. 

AGRO Acção 8.1 projecto 762. 2004 a 2008(PI) - EnoSafe – Segurança e controlo de qualidade de vinhos: implementação de técnicas rápidas para monitorização e rastreabilidade microbiológica. 

Fundação Calouste Gulbenkian. Março 2002 - Março de 2003: Desenvolvimento de protocolos experimentais com aplicação no ensino secundário nas áreas da microbiologia e da genética molecular: contribuição para a formação contínua de professores (PI)

 Acções Luso-Espanholas - 2005/06. Acção nº E-84/05

 Acções Bilateriais GRICES - CNPq Brasil 2005 – 2007

 Acordo ICCT/Embaixada de França – Programa 2002, Proc - 657 C2.7

 Acordo de Cooperação Luso/Francesa – Programa PESSOA 2004, Proc - 4.1.1/França.

TASK LEADER:

PTDC/EBB-EBI/109093/2008 - Chimera - Development of new chimeric proteins to be used in the fabrication of a novel biomaterial for bone regeneration application. Task Leader.

PTDC/SAU-MIC/119069/2010 -  Mecanismos de tolerância de biofilmes de Candida glabrata a agentes antifungicos. Task Leader.

apeamento estrutural e funcional das xilanases como ferramenta para optimização biotecnológica de uma xilanase altamente ativa da Antártida. Fundação para a Ciência e Tecnologia EXPL/BBB-BIO/1772/2013. € 43.590,00. Task Leader.

ACROSS-Desenvolvimento de suturas antimicrobianas à base de seda de aranha para infecções cirúrgicas. ACROSS-Development of AntimiCRObial Spider Silk sutures for preventing surgical site infections. PTDC/BBB-BIO/0827/2012. 01-01-2013 - 31-12-2014. Task Leader.

INTERREG III A Espanha-Portugal, de Maio 2006 a Setembro 2008– NaturPlas2 – Novos materiais com base em recursos naturais da euro-região: centro virtual de desenvolvimento de compósitos biodegradáveis

INTERREG III A Espanha-Portugal, NaturPlas – Novos materiais com base em recursos naturais da euro-região: aplicações na indústria automóvel

POCTI/ BIO/45356/2002. Funcionalização de materiais biomédicos usando um domínio de ligação a carbohidratos.

 POCI/BIA/BCM/57812/2004. Molecular biology of the monocarboxylic acids permeases in yeast: from functional to structural analysis. 

 POCI/AGR/56102/2004. Novas abordagens para a indústria de vinhos: exploração de recursos genéticos de leveduras e desenvolvimento de métodos de diagnóstico molecular

 TEAM MEMBER:

INNOYEAST– Innovative and improvement of European wine industry competetiveness by the research and development of native microcapsulated wine yeasts to produce quality wines. Programa: FP7-SME-2008-01- Research for SMEs.-

PTDC/BIA-BCM/64745/2006. Implementation of a National facility for DNA Microarrays: Phase II.

PTDC/EBB-BIO/104980/2008. BIOMYR: Towards the metabolic engineering of beta-myrcene pathway of Pseudomonas sp. M1: functional genomics and structural biochemistry approaches.

PTDC/QUI/69795/2006. MONOLIPO – Monoolein-based lipoplexes: Physicochemical characterization and study of their potential as non-viral vectors.

EUROPHEN - European Research Network for Yeast Phenomics. Programa: European Science Foundation RESEARCH NETWORK

EU/Agriculture and Agro-Industry, Including Fisheries (1993 - 1996), Spoilage yeasts in food and beverages: characterization and ecology for improved diagnosis and culture (CT93-0830)

POCI/AGR/56771/2004. Leveduras produtoras de fenóis voláteis: uma grande preocupação na indústria dos vinhos que necessita de ser compreendida

Fundação Calouste Gulbenkian de Março 2002 a Março de 2005: Transição, adaptação e sucesso académicos de alunos universitários

Ciência Viva - Agência Nacional para a Cultura Científica e Tecnológica (2006 - 2007), coordenado pela Drª Teresa Vieira da Escola Secundária Pluricurricular Santa Maria Maior

PAMAF 2029 - Projecto 2025 (1996 a 1999)

PRAXIS 2/2.1/BIO/1068/95- (1996 a 1999)

Glaxo Farmacêutica, de 1992 a 1995: Transporte de ácidos orgânicos e sua regulação em leveduras

COST- Project 87 - New Regeneration Techniques de 1987 a 1988.

JNICT (cont 819.86.03), de 1986 a 1989,A biotecnologia vegetal no melhoramento das castas do vinho verde. Aplicação de técnicas de cultura in vitro 

 

Publications

ResearcherID: B-6386-2009
http://www.researcherid.com/rid/B-6386-2009
http://scholar.google.pt/citations?user=02W9yO4GjtsC&;hl=pt-PT&oi=ao

2016

A-79. Azevedo-Silva J, Queirós O, Baltazar F, Ulaszewski S, Goffeau A, Ko Y H, Pedersen P L, Preto A, Casal M. 2016. The Anticancer Agent 3-Bromopyruvate: A Simple But Powerful Molecule Taken From The Lab To The Bedside. J Bioenerg Biomembr. epub ahead of print. doi: 10.1007/s10863-016-9670-z

A-80. Ferro, S., Azevedo-Silva, J., Casal, M., Côrte-Real, M., Baltazar, F., Preto, A. (2016). Characterization of acetate transport in colorectal cancer cells and potential therapeutic implications Oncotarget 7 (43), 70639-70653

A-81. Barroca, M., Rodrigues, P., Sobral, R., Costa, M.M., Chaves, S., Machado, R., Casal, M., Collins, T. 2016. Antibiotic free selection for the high level biosynthesis of a silkelastin-like protein. Scientific Reports. DOI: 10.1038/srep39329.

 

2015

 

A-72. da Costa, A., Machado, R., Ribeiro, A., Collins, T., Thiagarajan, V., Neves-Petersen, M.T., Rodríguez-Cabello, J.C., Gomes A.C., Casal, M.. 2015. Development of Elastin Like Recombinamer films with antimicrobial activity. Biomacromolecules 15, 625?635.

 

A-73. João Azevedo-Silva, Odília Queirós, Ana Ribeiro, Fátima Baltazar, Ko H Young, Peter L Pedersen, Ana Preto and Margarida Casal. 2015. The cytotoxicity of 3-Bromopyruvate in breast cancer cells depends on extracellular pH. Biochem. J. 467, 247–258.

 

A-74. Sá-Pessoa, J., Amillis, S., Casal *, M., Diallinas, G. * corresponding author. 2015. Expression and specificity profile of the major acetate transporter AcpA in Aspergillus nidulans. Fungal Genetics and Biology 76: 93–103.

 

A-75. Machado, R. da Costa, A., Sencadas, V., Pereira, M., Collins, T., Rodríguez-Cabello, J.C., Lanceros-Méndez, S., Casal, M. 2015. Exploring the properties of genetically engineered silk-elastin-like protein films Macromolecular Bioscience 15, 1698–1709

 

A-76. Mota S., Alves R., Carneiro C., Silva S., Brown A.J., Istel F., Kuchler K., Sampaio P., Casal M., Henriques M., Paiva S. 2015. Candida glabrata susceptibility to antifungals and phagocytosis is modulated by acetate. Frontiers in Microbiology. Vol 6. Article 919. doi: 10.3389/fmicb.2015.00919, 2015M

 

A-77. Padrão, J., Machado, R., Casal, M., Lanceros-Méndez, S., Rodrigues, L. R. Dourado, F., Sencadas, V. 2015. Antibacterial performance of bovine lactoferrin-fish gelatine electrospun membranes. International Journal of Biological Macromolecules 81: 608-614.

 

A-78. Soares-Silva I, Ribas D, Foskolou IP, Barata B, Bessa D, Paiva S, Queirós O, Casal M. 2015. The Debaryomyces hansenii carboxylate transporters Jen1 homologues are functional in Saccharomyces cerevisiae. FEMS Yeast Res. 15.

2014.

 

 A-69. Collins, T., Barroca, M., Branca, F., Padra?o, J., Machado, R. and Casal, M. 2014. High Level Biosynthesis of a Silk-Elastin-like Protein in E. coli. Biomacromolecules 15, 2701-8.

 A-70. Mota, S., Vieira, N., Barbosa, S., Delaveau, T., Torchet, C., Le Saux, A., Garcia, M., Pereira, A., Lemoine, S., Coulpier, F., Darzacq, X., Benard, L., Casal, M., Devaux, F. and Paiva, S. 2014. Role of the DHH1 gene in the Regulation of Monocarboxylic Acids Transporters Expression in Saccharomyces cerevisiae. PLoS ONE 9(11): e111589. doi:10.1371/journal.pone.0111589

A-71. Baltazar, F., Pinheiro, C., F. Morais- Santos, F., Azevedo-Silva, J., Queirós, O., Preto, A. and M. Casal. 2014. Monocarboxylate transporters as targets and mediators in cancer therapy response. Histology and Histopathology 29: 1511-1524.


2013

A-68. Machado, Raul; da Costa, André; Sencadas, Vitor; Garcia-Arévalo, Carmen; Costa, Carlos; Padrão, Jorge; Gomes, Andreia; Lanceros-Mendez, Senentxu; Rodríguez-Cabello, José Carlos; Casal, Margarida. 2013. Electrospun silk-elastin-like fiber mats for tissue engineering applications. Biomedical Materials (in press). IF: 2.174 (2012)

A-67. Siva, C., Matamá, T., Azoia, Nuno G., Mansilha, C., Casal, M. Cavaco-Paulo, A. 2013. Odorant binding proteins: a biotechnological tool for odour control. Appl Microbiol Biotechnol. 1-10. DOI 10.1007/s00253-013-5243-9. IF: 3.689 (2012)

A-66. Sá-Pessoa, J., Paiva, S., Ribas, D., Silva, I. J., Viegas, S. C., Arraiano, C., Casal, M. 2013. SatP (YaaH), a succinate-acetate transporter protein in Escherichia coli. Biochemical Journal Immediate. DOI:10.1042/BJ20130412. IF: 4.9 (2012)

A-65. Paiva, S., Strachotová, D., Kucerová, H., Hlavácek, O., Mota, S., Casal*, M, Palková, Z, Váchová, L. 2013. The transport of carboxylic acids and important role of Jen1p transporter during the development of yeast colonies. l Biochemical Journal 454: 551-8. *corresponding author / IF: 4.9 (2012)

A-64. Drumonde-Neves, J., Vieira, E., Lima, M.T., Araújo, I., Casal, M., Schuller, D. 2013. An easy, quick and cheap high-throughput method for yeast DNA extraction from microwell plates. Journal of Microbiological Methods 93: 206–208. IF: 2.161 (2012)

A-63. Collins, T., Azevedo-Silva, J., da Costa, A., Branca, F., Machado, R., Casal, M. 2013. Batch production of a silk-elastin-like protein in E. coli BL21(DE3): key parameters for optimisation. Microbial Cell Factories. 12: 21 doi:10.1186/1475-2859-12-21. IF: 3.306 (2012)

A-62. Machado, R., Azevedo-Silva, J., Correia, C., Collins, T., Arias, F. J., Rodríguez-Cabello, J.C., Casal, M. 2013 High level expression and facile purification of recombinant silk-elastin-like polymers in auto induction shake flask cultures. AMB Express Feb 5;3(1):11.) IF: waiting


2012
A-61. Becuwe, M, Vieira, N., Lara, D., Gomes-Rezende, J., Soares-Cunha, C., Haguenauer-Tsapis, R., Vincent, O., Casal, M., Paiva, S. and Léon, S. 2012. A molecular switch on an arrestin-like protein relays glucose signaling to transporter endocytosis. J. Cell Biology, 196, 247-55. IF: 10.822. 

A-60. Schuller, D., Cardoso, F., Sousa S., Gomes, P., Gomes, A.C., Santos, M. A. S., Casal, M. 2012. Genetic diversity and population structure of Saccharomyces cerevisiae strains isolated from different grape varieties and winemaking regions. PlosOne. Volume 7, Issue 2, e32507. IF: 3.730 

A-59. Matama, T., Casal, M., Cavaco-Paulo, A. 2012. Direct enzymatic esterification of cotton and Avicel with wild-type and engineered cutinases. Cellulose. DOI 10.1007/s10570-012-9827-9. 
IF = 3.476.

A-58. Pinheiro, C., Longatto-Filho, A., Azevedo-Silva, J., Casal, M., Schmitt, F.C., Baltazar, F. 2012. Role of monocarboxylate transporters in human cancers: state of the art (mini-review). J. Bioenerg. Biomembr. 44, 127-39. IF = 1.684 

A-57. Lis P, Zarzycki M, Ko YH, Casal M, Pedersen PL, Goffeau A, Ulaszewski S. 2012. Transport and cytotoxicity of the anticancer drug 3-bromopyruvate in the yeast Saccharomyces cerevisiae J. Bioenerg. Biomembr. 44, 155-61. IF = 1.684 

A-56. Queirós, O., Preto, A. Pacheco, A., Pinheiro, C., Azevedo-Silva, J., Moreira, R., Pedro, M., Ko, Young, Pedersen, Peter L., Baltazar, F., Casal, M. 2012. Butyrate activates the monocarboxylate transporter MCT4 expression in breast cancer cells and enhances the antitumor activity of 3-bromopyruvate. Journal of Bioenergetics and Biomembranes 44, 141-153. IF = 1.684 

A-55. Pacheco, A., Talaia, G., Sá-Pessoa, J., Bessa, D., Gonçalves, M.J., Moreira, R., Paiva, S., Casal, M., Queirós, O. 2012. Lactic acid production in Saccharomyces cerevisiae is modulated by expression of the monocarboxylate transporters Jen1 and Ady2. FEMS Yeast Research 12, 375-81. IF: 2.462.

A-54. Machado, R., Bessa, P.C., Reis, R. L. Reis, Rodriguez-Cabello, J.C. Casal, M. 2012. Elastin-Based Nanoparticles for Delivery of Bone Morphogenetic Proteins. Methods in Molecular Biology, 906: 353-63. ISBN 978-1-61779-952-5. IF:

A-53. Baleiras-Couto, M. M., Gomes, A.S., Casal, M., Duarte, F.L. 2012. Survey of yeast diversity during wine bottling processes using restriction analysis of 26S rDNA. Australian Journal of Grape and Wine Research 18, 39–42. (doi: 10.1111/j.1755-0238.2011.00167.x). IF: 2.958.

2011

A-52. Silva, C., Da, S., Silva, N.; Matama, T., Araujo, R., Martins, M., Chen, S., Chen, J., Wu, J., Casal, M., Cavaco-Paulo, A. 2011. Engineered Thermobifida fusca cutinase with increased activity on polyester substrates. Biotechnology Journal, 6: 1230-1239. DOI: 10.1002/biot.201000391. IF:3.446.

A-51. Silva JP, Oliveira AC, Casal MP, Gomes AC, Coutinho PJ, Coutinho OP, Oliveira ME. 2011. DODAB:Monoolein-based Lipoplexes as Non-viral Vectors for Transfection of Mammalian Cells. BBA – Biomembranes 1808:2440-9. IF=3.998.

A-50. Soares-Silva I, Sá-Pessoa J, Myrianthopoulos V, Mikros E, Casal M, Diallinas G. 2011. A substrate translocation trajectory in a cytoplasm-facing topological model of the monocarboxylate/H(+) symporter Jen1p. Mol Microbiol. 81:805-17. IF=5.010

A-49. Silva, C., Matamá, T., Kim, S.-Y, Padrão, J., Prasetyo, E. N., Kudanga T.,. Nyanhongo, G. S., Guebitz, G. M., Casal, M., Cavaco-Paulo, A. 2011. Antimicrobial and antioxidant linen via laccase-assisted grafting. Reactive & Functional Polymers 71: 713–720. IF: 2.479


2010
A-48. Bessa, P., Machado, R., Nürnberger , S., Dopler, D., Banerjee, A., Cunha, A. M., Rodríguez-Cabello, C., Redl, H., van Griensven, M., Reis, R.L., Casal, M. 2010. Thermoresponsive self-assembled elastin-based nanoparticles for delivery of BMPs. Journal of Controlled Release 142: 312–318. 
IF: 7.164


A-47. Vieira, N., Casal, M., Johansson, B., Paiva, S. MacCallum, D. M., Odds, F., Brown, A.J.P., Paiva, S. 2010. Functional specialization and differential regulation of short chain carboxylic acid transporters in the pathogen Candida albicans. Molecular Microbiology 75: 1337–1354. 
IF: 4.819

A-46. Bessa, P.C., Balmayor, E.R., Hartinger, J., Zanoni, G., Dopler, D., Meinl, A., Banerjee, A., Casal, M., Redl, H., Reis, R.L., van Griensven, M. 2010. Silk fibroin microparticles as carriers for delivery of human recombinant BMP-2: In vitro and in vivo bioactivity. Tissue Engineering Part C Methods 4: 349-55.
IF: 4.839

A-45. Vieira, N., Pereira, F., Casal, M., Brown, A.J.P., Paiva, S., Johansson, B. 2010. Plasmids for in vivo construction of integrative Candida albicans vectors in Saccharomyces cerevisiae. Yeast 27:933-9. 
IF: 1.626 

A-44. Moreira S, Castanheira P, Casal M, Faro C, Gama M. 2010. Expression of the functional Carbohydrate-Binding Module (CBM) of human laforin. Protein Expr Purif. 74:169-74. 
IF: 1.644

A-43. Matamá, T., Araújo, R., Guebitz, G. Casal, M., Cavaco-Paulo, A. 2010. Functionalization of cellulose acetate fibers with engineered cutinases. Biotechnology Progress 26: 636-43. 
IF: 2.170

A-42. Bessa, P.C., Balmayor, E.R, Azevedo, H.S., Nürnberger, S., Casal, M., van Griensven, M., Reis, R.L., Redl, H. 2010. Silk fibroin microparticles as carriers for delivery of human recombinant BMPs. Physical caracterization and drug release. Journal of Tissue Engineering and Regenerative Medicine 4 349-55. 
IF: 4.839

2009
A-41. Araújo, R., Silva, C., Machado, R., Casal, M., Cunha, A.M., Rodriguez-Cabello, C., Cavaco-Paulo, A. 2009. Proteolytic enzyme engineering: a tool for wool. Biomacromolecules 10, 1655-61
IF: 4.502

A-40. Paiva, S. Vieira, N., Nondier, I., Haguenauer-Tsapis, R., Casal, M., Urban-Grimal, D. 2009. Glucose-induces ubiquitylation and endocytosis of the yeast Jen1 transporter: role of ubiquitin-K63 chains. J. Biol.Chem. 284, 19228-36.
IF: 5.532 

A-39. Machado, R., Ribeiro, A. J., Padrão, J., Silva, D., Nobre, A., Teixeira, J.A. Cunha, A. M., Rodríguez-Cabello, J. C., Casal, M. 2009. Exploiting the sequence of naturally occurring elastin: Construction, production and characterization of a recombinant thermoplastic protein-based polymer. Journal of Nano Research 6, 133-145.
IF: 0,571

A-38. Bessa, P.C., Cerqueira, M.T., Rada, T., Gomes, M.E., Neves, N.M., Nobre, A., Reis, R.L., Casal, M. 2009. Expression, purification and osteogenic bioactivity of recombinant human BMP-4, -9, -10, -11 and -14. Protein Expression and Purification 63, 89-94. 
IF: 1.621

2008

A-37. Casal, M., Paiva, S., Queirós, O., Soares-Silva, I. 2008. Transport of carboxylic acids in yeasts. FEMS Microbiology Reviews. 32, 974–994. 
IF: 7.963 (top 10% in Microbiology)

A-36. Araújo, R., Cavaco-Paulo, A., Casal, M. 2008. Strategies Towards the functionalization of Bacillus subtilis subtilisin E for wool finishing applications. Engineering in Life Sciences 8, 238-249. IF: 1.200

A-35. Araújo, R., Casal, M., Cavaco-Paulo A. 2008. Application of enzymes for textile fibres processing. Biocatalysis and Biotransformation 26, 332-349. IF: 1.170

A-34. Bessa, P.C., Casal, M., Reis, R.L. 2008. Bone morphogenetic proteins in TE: the road from laboratory to the clinic, part II (BMP delivery). Journal of Tissue Engineering and Regenerative Medicine 2, 81-96. IF: 1.615 

A-33. Bessa, P.C., Casal, M., Reis, R.L. 2008. Bone morphogenetic proteins in TE: the road from laboratory to the clinic, part I (basic concepts). Journal of Tissue Engineering and Regenerative Medicine 2, 1-13. IF: 1.615 

A-32. Oliveira, V.A., Vicente, M.A., Fietto, L.G., Castro, I.M., Coutrim, M.X., Schuller, D., Casal. M., Santos, J.O., Araújo, L.D., Silva. P.H.A., Brandão, R.L. 2008. Biochemical and molecular characterization of Saccharomyces cerevisiae strains obtained from sugar-cane juice fermentations and their impact in “cachaça” production. Applied Microbiology and Biotechnology 74, 693-701. IF: 2.569

A-31. Bessa, P.C., Pedro, A.J., Klosch, B., Nobre, A., van Griensven, M., Reis, R.L., M. Casal. 2008. Osteoinduction in human fat derived stem cells by recombinant human bone morphogenetic protein-2 produced in Escherichia coli. Biotechnology Letters 30, 15–21. IF: 1.595

2007-1995

A-30. O´Neill, N., Araújo, R., Casal, M., Guebitz, G., Cavaco-Paulo, A. 2007. Effect of the agitation on the adsorption and hydrolytic efficiency of cutinases on polyethylene terephthalate fibres. Enzyme and Microbial Technology 40, 1801–1805. IF: 1.969
A-29. Schuller, D., Pereira, L., Alves, H., Cambon, B., Dequin, S., Casal, M. 2007. Genetic characterization of commercial Saccharomyces cerevisiae isolates recovered from vineyard environments. Yeast 24, 625-636 IF: 2.619
A-28. Soares-Silva, I., Paiva, S., Diallinas, G., Casal, M. 2007. The conserved sequence NXX[S/T]HX[S/T]QDXXXT of the lactate/pyruvate:H+ symporter subfamily defines the function of the substrate translocation pathway. Molecular Membrane Biology 24, 464-474. IF: 3.870
A-27. Araújo, R., O´Neill, A., Micaelo, N., Soares, C., Fischer-Colbrie, G., Guebitz, G., Casal, M., Cavaco-Paulo, A. 2007. Tailoring cutinase activity towards polyethylene terephthalate 6,6 fibers. Journal of Biotechnology 128, 849–857. IF: 2.565
A-26. Queiros, O., Pereira, L., Paiva, S., Moradas-Ferreira, P., Casal, M. 2007. Functional analysis of Kluyveromyces lactis carboxylic acids permeases: heterologous expression of KlJEN1 and KlJEN2 genes. Current Genetics 51, 161-169. IF: 2,507
A-25. Silva, C., Araújo, R., Casal, M., Guebitz, G., Cavaco-Paulo, A. 2007. Influence of mechanical agitation on cutinases and protease activity towards polyamide substrates. Enzyme and Microbial Technology 40, 1678–1685. IF: 1,969
A-24. Valero, E., Cambon, B., Schuller, D., Casal., M., Dequin, S. 2007. Biodiversity of Saccharomyces yeast strains from grape berries of wine-producing areas using starter commercial yeasts. FEMS Yeast Res. 7, 317-29. IF: 2,812
A-23. Schuller, D., Casal, M. 2007. The genetic structure of fermentative vineyard-associated Saccharomyces cerevisiae populations revealed by microsatellite analysis. Antonie van Leeuwenhoek International Journal of General and Molecular Microbiology 91, 137-150. IF: 1,547 
A-22. Schuller, D., Casal, M. 2005. The use of genetically modified yeast in the wine industry (review). Applied Microbiology and Biotechnology 68, 292–304. IF: 2,586 
A-21. Andrade, R.P., Kötter, P., Entian, K.-D., Casal, M. 2005. Multiple transcripts regulate glucose-triggered mRNA decay of the lactate transporter JEN1 from Saccharomyces cerevisiae. Biochemical and Biophysical Research Communications 332, 254–262. IF: 3,000 
A-20. Valero, E., Schuller, D., Cambon, B., Casal, M., Dequin, S. 2005. Dissemination and survival of commercial wine yeast in the vineyard: a large-scale, three years study. FEMS Yeast Res. 5, 959–969. IF: 2,477 
A-19. Gomes, K.N., Freitas, S.M.A.C., Pais, T.M., Fietto, J.L.R., Totola, A.H., Arantes, R.M.E., Martins, A., Lucas, C., Schuller, D., Casal, M., Castro, I.M., Fietto, L.G., Brandão, R.L. 2005. Deficiency of Pkc1 activity affects glycerol metabolism in Saccharomyces cerevisiae. FEMS Yeast Res. 5, 767–776. IF: 2,477 
A-18. Ramalho, P.A., Paiva, S., Cavaco-Paulo, A., Casal, M., Cardoso, M.H., Ramalho, M.T. 2005. Azo reductase activity of intact Saccharomyces cerevisiae cells is dependent on the Fre1p component of plasma membrane ferric reductase. Applied and Environmental Microbiology 71, 3882–3888. IF: 3,818 
A-17. Schuller, D., Alves, H., Dequin, S., Casal, M. 2005. Ecological survey of Saccharomyces cerevisiae strains from vineyards in the Vinho Verde Region of Portugal. FEMS Microbiology Ecology 51, 167-177. IF: 2,787 
A-16. Soares-Silva, I., Paiva, S., Kötter, P., Entian, K.-D., Casal, M. 2004.The disruption of JEN1 from Candida albicans impairs the transport of lactate. Molecular Membrane Biology 21, 403-411. IF: 5,667 
A-15. Paiva, S., Devaux, F., Barbosa, S., Jacq, C., Casal, M. 2004. Ady2p is essential for the acetate permease activity in the yeast Saccharomyces cerevisiae. Yeast 21, 201-210. 
A-14. Schuller, D., Valero, E., Dequin, S., Casal, M. 2004. Survey of molecular methods for the typing of wine yeast strains. FEMS Microbiology Letters 231, 19-26. 
A-13. Soares-Silva, I., Schuller, D., Andrade, R.P., Baltazar, F., Cássio, F., Casal, M. 2003. Functional expression of the lactate permease Jen1p of Saccharomyces cerevisiae in Pichia pastoris. Biochemical Journal 376, 781-787. 
A-12. Salgado, A.P.C., Schuller, D., Casal, M., Leão, C., Leiper, F.C., Carling, D., Fietto, L.G., Trópia, M.J., Castro, I.M., Brandão, R.L. 2002. Relationship between protein kinase C and derepression of different enzymes. FEBS Letters 532, 324-33 2.
A-11. Paiva, S., Kruckeberg, A., Casal, M. 2002. Utilization of green flourescent protein as a marker for studying the expression and turnover of the monocarboxylate permease JEN1p of Saccharomyces cerevisiae. Biochemical Journal 363, 737-744.
A-10. Queirós, O., Casal, M., Moradas-Ferreira, P., Leão, C. 2001. PCR –based method for the construction of gene disruption cassettes in yeast. Biotechniques 31, 264-266.
A-9. Makuc, J., Paiva, S., Schauen, M., Krämer, R., André, B., Casal, M., Leão, C., Boles, E. 2001. The putative monocarboxylate permeases of the yeast Saccharomyces cerevisiae do not transport monocarboxylic acids across the plasma membrane. Yeast 18, 1131-1143.
A-8. Andrade, R.P., Casal, M. 2001. Expression of the lactate permease gene Jen1 from the yeast Saccharomyces cerevisiae. Fungal Genetics and Biology 32, 105-111.
A-7. Casal, M., Paiva, S., Andrade, R.P., Gancedo, C., Leão, C. 1999. The lactate-proton symport of Saccharomyces cerevisiae is encoded by JEN1. Journal of Bacteriology 181, 2620-2623.
A-6. Paiva, S., Althoff, S., Casal, M., Leão, C. 1999. Transport of acetate in mutants of Saccharomyces cerevisiae defective in monocarboxylate permeases. FEMS Microbiology Letters 170, 301-306.
A-5. Queirós, O., Casal, M., Althoff, S., Moradas-Ferreira, P., Leão, C. 1998. Isolation and characterization of Kluyveromyces marxianus deficient mutants on malate transport. Yeast 14, 401-407.
A-4. Casal, M., Cardoso, H., Leão, C. 1998. Effects of ethanol and other alkanols on the transport of acetic acid in Saccharomyces cerevisiae. Applied and Environmental Microbiology 64, 665-668.
A-3. Casal, M., Cardoso, H., Leão, C. 1996. Mechanisms regulating the transport of acetic acid in Saccharomyces cerevisiae. Microbiology 142, 1385-1390.
A-2. Casal, M., Blázquez, M., Gamo, F.J., Gancedo, C., Leão, C. 1995. Lack of lactate-proton symport activity in pck1 mutants of Saccharomyces cerevisiae. FEMS Microbiology Letters 128, 279-282.
A-1. Casal, M., Leão, C. 1995. Utilization of short-chain monocarboxylic acids by the yeast Torulaspora delbrueckii: specificity of the transport systems and their regulation. Biochimica et Biophysica Acta - Molecular Cell Research 1267, 122-130.

P-1. Pais, M. S. S., Casal, M. 1987. Propagation of the fern Adiantum capillus-veneris through tissue culture of the circinate part of young leaves. Acta Horticulturae, 212, 651-65. Symposium In Vitro Problems Related To Mass Propagation of Horticultural Plants. Gembloux, Bélgica, 1985.

P-2. Casal, M. e Pais, M. S. S. 1987. The effect of benzyladenine and naphthalene-acetic acid on in vitro culture of Vitis vinifera (cv. Loureiro). Ciência Biológica, 12 (5A): 141. Livro de resumos do 8º Congresso Nacional de Bioquímica. Póvoa de Varzim.

P-3. Casal, M., Leão, C. 1994. Utilization of acetic acid and other short-chain monocarboxylic acids in the yeasts Torulaspora delbrueckii and Saccharomyces cerevisiae: transport and its regulation. Folia Microbiologica (Praha), 39/6, 512. Book of Abstracts of the XII Small Meeting on Yeast Transport and Energetics (SMYTE). Karpacz, Poland.

P-4. Casal, M., Blázquez, M., Gamo, F.-J., Gancedo, C., Leão, C. 1994. Transport of lactate and its regulation in Saccharomyces cerevisiae deficient mutants on specific steps of the metabolism. Folia Microbiologica (Praha), 39/6, 512. Book of Abstracts of the XII Small Meeting on Yeast Transport and Energetics (SMYTE). Karpacz, Poland.

P-5. Casal, M., Queirós, O., Moradas-Ferreira, P., Leão, C. 1995. Isolation and characterization of Kluyveromyces marxianus deficient mutants on malate transport. Yeast, 11. Book of Abstracts of the XVII International Conference on Yeast Genetics and Molecular Biology. Lisboa, Portugal.

P-6. Casal, M., Blázquez, M., Gamo, F.-J., Gancedo, C., Leão, C. 1995. Lack of lactate-proton symport activity in pck1 mutants of Saccharomyces cerevisiae. Yeast, 11. Book of Abstracts of the XVII International Conference on Yeast Genetics and Molecular Biology. Lisboa, Portugal.

P-7. Queirós, O., Casal, M., Althoff, S., Moradas-Ferreira, P., Leão, C. 1998. Phenotypic characterisation of Kluyveromyces marxianus deficient mutants on malate transport. Folia Microbiologica (Praha), 43/2. Book of Abstracts of the XV Small Meeting on Yeast Transport and Energetics (SMYTE). San José de Vistahermosa, Morelos, México.

P-8. Paiva, S., Casal, M., Leão, C. 1999. Genetic characterization of monocarboxylic acids transporters of Saccharomyces cerevisiae. Folia Microbiologica (Praha), 44/2, 232-233. Book of Abstracts of the XVI Small Meeting on Yeast Transport and Energetics (SMYTE). Bratislava, Eslováquia.

P-9. Queirós, O., Casal, M., Moradas-Ferreira, P., Leão, C. 1999. Engineering of a Saccharomyces cerevisiae strain improves the ability to use malic acid. Current Genetics, 35, nº 3-4 (208). Book of Abstracts of the XIX International Conference on Yeast Genetics and Molecular Biology. Rimini, Itália.

P-10. Casal, M., Paiva, S., Andrade, R.P., Leão, C. 1999. Genetic and functional analysis of the Saccharomyces cerevisiae gene Jen1 encoding the lactate permease. Current Genetics, 35, nº 3-4 (182). Book of Abstracts of the XIX International Conference on Yeast Genetics and Molecular Biology. Rimini, Itália.

P-11. Andrade, R.P., Casal, M. 2001. Catabolite regulation of Saccharomyces cerevisae JEN1 expression: targeting transcription and mRNA decay. E.J.B., 268/1, 100 (PS3-173). Book of Abstracts of the 27th Meetting of the FEBS and Pan-American Association Molecular Biology. Lisboa, Portugal.

P-12. Makuc, J., Paiva, S., Schauen, M., Krämer, R., André, B., Casal, M., Leão, C.  Boles, E. 2001. The putative monocarboxylate permeases of the yeast Saccharomyces cerevisiae do not transport monocarboxylic acids across the plasma membrane. E.J.B., 268/1, 53 (PS1-053). Book of Abstracts of the 27th Meetting of the FEBS and Pan-American Association Molecular Biology. Lisboa, Portugal.

P-13. Queirós, O., Casal, M., Moradas-Ferreira, P., Leão, C. 2001. Molecular studies on malic acid transport in the yeast Kluyveromyces marxianus. E.J.B., 268/1, 266 (POTH-052). Book of Abstracts of the 27th Meetting of the FEBS and Pan-American Association Molecular Biology. Lisboa, Portugal.

P-14. Paiva, S., Kruckeberg, A., Casal, M. 2001. Catabolite inactivation of the lactate transporter in Saccharomyces cerevisiae: ubiquitination, endocytosis and degradation in the vacuole. Yeast, 18 (S251). Book of Abstracts of the XX International Conference on Yeast Genetics and Molecular Biology. Praga. República Checa.

P-15. Andrade, R. P., Casal, M. 2001. Saccharomyces cerevisiae JEN1 expression: catabolite regulation of transcription and mRNA turnover. Yeast, 18 (S85). Book of Abstracts of the XX International Conference on Yeast Genetics and Molecular Biology. Praga. República Checa.

P-16. Queirós, O., Casal, M., Moradas-Ferreira, P., Leão, C. 2001. Molecular studies on a Kluyveromyces marxianus malate inducible plasma membrane protein. Yeast, 18 (14-35). Book of Abstracts of the XX International Conference on Yeast Genetics and Molecular Biology. Praga. Republica Checa.

P-17. Soares-Silva, I., Andrade, R.P., Schuller, D., Casal, M. 2003. Exploring genetic tools for the over-expression of the lactate permease Jen1p of Saccharomyces cerevisiae: constitutive expression in S. cerevisiae and heterologous expression in Pichia pastoris. Yeast, 20 (S246). Book of Abstracts of the XXI Yeast Genetics and Molecular Biology Meeting. Gothenburg, Suécia.

P-18. Paiva, S., Soares-Silva, I., Kötter, P., Entian, K.-D., Casal, M. 2003. The disruption of JEN1 from Candida albicans impairs the transport of lactate. Yeast, 20 (S242). Book of Abstracts of the XXI Yeast Genetics and Molecular Biology Meeting. Gothenburg, Suécia.

P-19. Queirós, O., Paiva, S., Moradas-Ferreira, P., Casal, M. 2003. Molecular and physiological characterization of monocarboxylic acids permeases in the yeast Kluyveromyces lactis. Yeast, 20 (S237). Book of Abstracts of the XXI Yeast Genetics and Molecular Biology Meeting. Gothenburg, Suécia.

P-20. Valero, E., Schuller, D., Cambon, B., Casal, M., Dequin, S. 2005. Dissemination and survival of commercial wine yeast in the vineyard: a large-scale, three years study. Yeast, 22 (S50). Book of Abstracts of the XXII Yeast Genetics and Molecular Biology Meeting. Bratislava, Eslováquia.

P-21. Schuller, D., Alves, H., Cambon, B., Dequin, S., Casal, M. 2005. Genetic instability of a commercial Saccharomyces cerevisiae strain. Yeast, 22 (S208). Book of Abstracts of the XXII Yeast Genetics and Molecular Biology Meeting. Bratislava, Eslováquia.

P-22. Schuller, D., Sampaio, P., Pais, C., Casal, M. 2005. Genetic structure of vineyard-associated Saccharomyces cerevisiae populations revealed by microsatellite analysis. Yeast, 22 (S49). Book of Abstracts of the XXII Yeast Genetics and Molecular Biology Meeting. Bratislava, Eslováquia.

P-23. Valero, E., Schuller, D., Cambon, B., Casal, M., Dequin, S. 2005. Selected yeast utilization and biodiversity. Proceedings of the XVII Entretiens Scientifiques Lallemand: Yeast´s contribution to the sensory profile of wine, pp 55-60. La Rioja, Espanha.

P-24. Bessa, P.C., Pedro, A.J., Casal, M., Reis, R.L. 2005. Production of biologically active recombinant human BMP-2 for tissue engineering applications. The International Journal of Artificial Organs. Vol. 28, pp302. Abstracts of the 32nd ESAO –IFAO joint congress, Bolonha, Itália.

P-25. Dellinger, B., Graça, M.S., Casal, M., Schuller, D. 2007. Identification of wine related yeast species by capillary electrophoresis single- strand conformation polymorphism. Proceedings do congresso ŒNO 2007, le 8ème Symposium International d'Œnologie de Bordeaux, 25.06. – 27.06.2007, Bordeaux, França.

P-26. Balmayor, E.R., Bessa, P.C., Tuzlakoglu, K., Azevedo, H.S., Casal, M., Reis, R.L. 2008. Novel biodegradable polymeric microparticles for the localized delivery of differentiation agents in bone tissue engineering applications. Tissue Engineering, Part A, Vol. 14. Issue 5, Pages: 762-763. Meeting Abstract: OP210.

P-27. Bessa, P.C., Balmayor, E.R., Cerqueira, M.T., Rada, T., Gomes, M.E., Neves, N.M.,, Azevedo, H.S., Casal, M., Reis, R.L. 2008 Silk nanoparticles for delivery of human BMP-2 in bone regenerative medicine applications. Tissue Engineering, Part A, Vol. 14. Issue 5, Pages: 776-777. Meeting Abstract: OP253.

P-28. Moreira, S., Domingues, L., Casal, M., Gama, M. 2008. Development of a strategy to functionalize dextrin-based hydrogel for animal cell cultures. Tissue Engineering, Part A, Vol. 14. Issue 5, Pages 835-836. Meeting Abstract: P116.

P-29. Bessa, P.C., Cerqueira, M.T., Rada, T., Gomes, M.E., Neves, N.M., Casal, M., Reis, R.L. 2008. Expression, purification and bioactivity of recombinant human bone morphogenetic protein-4,-9,-10,-11 and-14 produced in Escherichia coli for tissue engineering applications. Tissue Engineering, Part A, Vol. 14. Issue 5, Pages: 852-852. Meeting Abstract: P168.

P-30. Vieira N., Johansson B., MacCallum D., Odds F., Casal M., Brown A. and Paiva S. Functional specialization and differential regulation of short chain carboxylic acid transporters in the pathogen Candida albicans. YEAST, Volume 26 Issue S1 , Pages S19 - S259 (July 2009), Page: 174. Supplement: Abstracts of the 24th International Conference on Yeast Genetics and Molecular Biology, Manchester, UK, 19-24 July 2009.

P-31. Strachotova, D, Paiva, S. Vachova, L., Casal, M., Palkova, Z. Sok2p plays a role in lactate/lactic acid uptake in yeast colonies. YEAST, Volume 26 Issue S1, Pages S19 - S259 (July 2009), Page: 226. Supplement: Abstracts of the 24th International Conference on Yeast Genetics and Molecular Biology, Manchester, UK, 19-24 July 2009.

P-32. Soares-Silva, I., Foskolou, Z., Diallinas, G., and Casal, M., Identification of a new domain of the lactate/pyruvate:H+ symporter subfamily critical for distinguishing mono- and di-carboxylate permeases. YEAST, Volume 26 Issue S1 , Pages S19 - S259 (July 2009), Page: 230. Supplement: Abstracts of the 24th International Conference on Yeast Genetics and Molecular Biology, Manchester, UK, 19-24 July 2009.

P-33. Diallinas, G., Soares-Silva, I., Sá Pessoa, J., Myrianthopoulos, V., Mikros, E., Casal, M. 2011. Identification of a Substrate Translocation Trajectory in the Inward-Facing Conformation of the Monocarboxylate/H+ Symporter Jen1. Biophysical Journal, Volume 100, Issue 3, Supplement 1, Page 246a.

P-34. Machado, R., García-Arévalo, C., Arias, F.J., Rodríguez-Cabello, J.C., Casal, M. 2011. Tunable morphology and structural properties of recombinant silk-elastinlike biopolymers by electrospinning. Biophysical Journal, Vol. 100. Issue 3, Supplement 1, Page 369a.

P-35. R. Franco-Duarte, L. Carreto, B. Cambon, S. Dequin, M. Santos, M. Casal, D. Schuller. Genetic characterization of commercial Saccharomyces cerevisiae isolates recovered from vineyard environments using comparative genome hybridization on array (aCGH). 2011. Yeast, 28, Supplement 1, S173.

http://hdl.handle.net/1822/13466

P-36. Léon, S., Becuwe, M., Vieira N., Lara, D., Casal, M., Vincent, O., Paiva, S. e Haguenauer-Tsapis, R. (2011). Connecting glucose signalling to ubiquitin-dependent transporter endocytosis: Arrestin the culprit!. 25th International Conference on yeast Genetics and Molecular Biology. Olsztyn, Polónia. 11-16 Julho 2011. Yeast Vol 28, Supp 1.

P-37. Vieira N., Pereira F., Paiva, S., Casal M., Brown A. e Johansson B. Plasmids for in vivo construction of integrative Candida albicans vectors in Saccharomyces cerevisiae”. 25th International Conference on Yeast Genetics and Molecular biology. Olsztyn, Polónia. 11-16 Julho 2011. Yeast Vol 28, Supp 1.

 P-38. E. Vieira, J. Drumonde-Neves, R. Machado, P. Silva, A.C. Gomes, S. Sousa, P.T. Ramos, F. Alemão, M.T.Lima, I. Araújo, F.L. Duarte, M.A. Santos, M. Casal e D. Schuller. A colecção de estirpes autóctones de Saccharomyces cerevisiae das principais Regiões Vitivinícolas Portuguesas. 8º Simpósio de Vitivinicultura do Alentejo, 5.5. – 7.5.2010, Évora, Portugal.  http://hdl.handle.net/1822/12029

P-39. A Pacheco, O Queirós, A Preto, C Pinheiro, J Azevedo-Silva, R Moreira, M Pedro, F Baltazar, M Casal. 3-bromopyruvate cytotoxic effect in breast cancer cells is dependent of monocarboxylate transporters (MCT) expression and is enhanced by butyrate. Exp Clin Endocrinol Diabetes 2012; 120 - A22. DOI: 10.1055/s-0032-1330817.

P-40. E Silva, M Casal, S Paiva, O Queirós. Subcellular localization of the monocarboxylate transporter Mct1 tagged with the green fluorescence protein in different cancer cell lines. Exp Clin Endocrinol Diabetes 2012; 120 - A30. DOI: 10.1055/s-0032-1330825. 

B-1. Casal, M., Pais, M. S. S. 1989. Micropropagation of Vitis vinifera from Vinho Verde Region of Portugal. A method for grapevine leafroll virus elimination. In, Plant Aging: Basic and Applied Approaches. Editores: R. Rodríguez, R. Sánches Tamés e D. J. Durzan. NATO ASI Series A: Life Science, Vol. 186, pp. 327-333. Plenum Press.

B-2. Queirós, O., Casal, M., Moradas-Ferreira, P., Leão, C. 2003. PCR-based strategy for gene disruption in Kluyveromyces lactis. In, Lab Manual on Non-conventional Yeasts Genetics, Biochemistry, Molecular Biology and Biotechnology. Editores: K. Wolf, K. Breunig, G. Barth. Chapter 5, pp 161-167. Springer Lab Manual.

B-3. Leonor, I.B., Gomes, S., Bessa, P.C., Mano, J.F., Casal, M., Reis, L.R. 2008. New biomineralization strategies for the use of natural-based polymeric materials in bone-tissue engineering. In, Natural-based polymers for biomedical applications. Eds, R L Reis, N M Neves, J F Mano, M E Gomes, A P Marques and H S Azevedo. Woodhead Publishing Limited, Cambridge, 193-230.

B-4.Araújo, R., Casal, M., Cavaco-Paulo, A. 2010. Design and engineering of novel enzymes for textile applications. In, Advances in Textile Biotechnology. Editores: V Nierstrasz, Ghent University, Belgium and A Cavaco-Paulo, University of Minho, Portugal Woodhead Textiles Series No. 107.

B-5. Castanheira, P., Moreira, S., Casal, M., Gama, M., Faro, C. 2011. Recombinant Laforin for Structural Studies. In, Underlying Mechanisms of Epilepsy. ISBN 978-953-307-765-9. Edited by: Fatima Shad Kaneez. Publisher: InTech, September 2011

B-6. Machado, R., Bessa, P.C., Reis, R. L. Reis, Rodriguez-Cabello, J.C. Casal, M. 2012. Elastin-Based Nanoparticles for Delivery of Bone Morphogenetic Proteins. In, Nanoparticles in Biology and Medicine. Methods and Protocols Series: Methods in Molecular Biology, Vol. 906. Soloviev, Mikhail (Ed.), Humana Press, ISBN 978-1-61779-952-5.

B-7. Casal, M., Cunha, A. M., Machado, R. 2013. Recombinant Protein-Based Polymers: the case study of future trends for silk-elastin-like polymers development and application. In, Bio-based Plastics: Materials and Applications. Published by: John Wiley & Sons Ltd.. Stephan Kabasci (Editor), Christian Stevens (Series Editor). ISBN: 978-1-119-99400-8

B-8. Casal, M., Queiros, O., Talaia, G., Ribas, D., Paiva, S. 2016. Carboxylic Acids Plasma Membrane Transporters in Saccharomyces cerevisiae. In, YEAST MEMBRANE TRANSPORT. Advances in Experimental Medicine and Biology. Volume: 892, pp 229-251. DOI: 10.1007/978-3-319-25304-6_9.

 

 

K-1. Filipa Carneiro, Carla Silva, Teresa Matamá, Rita Araújo, Margarida Casal, Georg Güebitz, Artur Cavaco-Paulo. CUTINASE PARA MODIFICAÇÃO DAS FIBRAS DE POLIACRILONITRILO E POLIAMIDA.              Patente Portuguesa n.º 103035.

K-2. Filipa Carneiro, Carla Silva, Teresa Matamá, Rita Araújo, Margarida Casal, Georg Güebitz, Artur Cavaco-Paulo. METHOD FOR THE MODIFICATION OF POLYACRYLONITRILE FIBRES CONTAINING VINYL ACETATE AS A COMONOMER AND POLYAMIDE FIBRES, USING A CUTINASE ENZYME.              International Publication Number WO 2005/040487 A1.

K-3. Moreira, S., Domingues, L., Casal, M. & Gama, M. PROTEÍNAS MODIFICADAS COMPREENDENDO UM PÉPTIDO/ PROTEÍNA BIOACTIVO(A) LIGADO(A) A UMA SEQUÊNCIA DE AMINOÁCIDOS QUE CONTÉM UM MÓDULO HUMANO DE LIGAÇÃO A CARBOHIDRATOS (CBM), E DESENVOLVIMENTO DE UM SISTEMA DE ADMINISTRAÇÃO DE PROTEÍNAS TERAPEUTICAMENTE ACTIVAS E RESPECTIVAS UTILIZAÇÕES PARA FINS BIOMÉDICOS.              Patente Portuguesa Nº 40724J. 2006.

K-4. Moreira, S., Domingues, L., Casal, M. & Gama, M. MODIFIED PROTEINS COMPRISING A BIOACTIVE PEPTIDE/ PROTEIN LINKED TO A SEQUENCE OF AMINOACIDS CONTAINING A HUMAN CARBOHYDRATE BINDING MODULE (CBM), AND DEVELOPMENT OF A SYSTEM FOR THE ADMINISTRATION OF THERAPEUTICALLY ACTIVE PROTEINS AND RESPECTIVE UTILIZATIONS FOR BIOMEDICAL PURPOSES.              International Publication Number WO 2007/072461.

K-5. Maria Elisabete C. D. Real Oliveira, Paulo J. G. Coutinho, Olga M. F. Pereira Coutinho, Andreia F. C. Gomes, Margarida P. P. Amorim Casal, João P. N. Silva. Aplicação da Monooleína como novo lípido adjuvante em vectores genéticos não-virais com potencial interesse nos domínios da Terapia Génica e Biologia Molecular. 2008.              Pedido de Patente Portuguesa Nº PN 104158.

K-6. Maria Elisabete C. D. Real Oliveira, Paulo J. G. Coutinho, Olga M. F. Pereira Coutinho, Andreia F. C. Gomes, Margarida P. P. Amorim Casal, João P. N. Silva. Aplicação da Monooleína como novo lípido adjuvante em lipofecção. Pedido de Patente Internacional PPI 40759/09.   International Patent in Submission, W.I.P. Organization, W0/2010/029035 A2, 25 Fev 2010.

K-7. Processo de tratamento de fibras de animais de forma a aumentar a resistência das mesmas ao encolhimento. 2008.              Pedido de Patente Portuguesa Nº PN 104124 H.

K-8. PROCESS FOR THE TREATMENT OF ANIMAL FIBRES AIMED AT IMPROVING THE SHRINK-RESISTANCE THEREOF. Processo de tratamento de fibras de animais de forma a aumentar a resistência das mesmas ao encolhimento. International Publication Number WO 2010/001356 A1

O-1. Casal, M., Leão, C. 1997. Construction of improved strains of Saccharomyces cerevisiae with capacity to remove weak acids during an alcoholic fermentation. Livro de Actas do XXII Congrés de la Vigne et du Vin, OIV. Buenos Aires, Argentina, Livro 2 – Enologia.

O-2. Casal, M. 2000. Contribuições para o estudo da permease de ácido láctico e de outros ácidos monocarboxílicos na levedura Saccharomyces cerevisiae. Boletim da Sociedade Portuguesa de Microbiologia nº 5, 11-18.

O-3. Leão, C., Rodrigues, F., Paiva, S., Queirós, O., Schuller, D., Cássio, F., Cardoso, H., Sousa, M. J., Casal, M., Côrte-Real, M. 2000. Remoção zimológica da acidez volátil e fixa de mostos e vinhos: da fisiologia à manipulação genética de leveduras. Boletim de Biotecnologia, nº 66, 7-13.

O-4. Schuller, D., Valero, E., Cambon, B. Casal, M., Dequin, S. Commercial yeast strains do not disseminate significantly in France and Portugal winery ecosystem. LXXXIII General Assembly of the International Office on Vine and Wine “The vitivinicole sector and biotechnologies: perspectives, stakes and risk evaluation”, Paris, 16-19 June, 2003. Livro de actas (formato CD-ROM).

O-5. Schuller, D., Alves, H., Dequin, S., Casal, M. 2005. Panorama ecólogico de cepas de Saccharomyces cerevisiae en un viñedo de la región vinho verde en Portugal. www.infowine.com, Revista Internet de Viticultura e Enologia, Nº 10/2.

O-6. Schuller, D., Alves, H., Dequin, S., Casal, M. 2005. Recensement ecologique des souches de Saccharomyces cerevisiae presentes dans des vignobles de la region du Vinho Verde (Portugal). www.infowine.com, Revista Internet de Viticultura e Enologia, Nº 10/2.

O-7. Schuller, D., Alves, H., Dequin, S., Casal, M. 2005. Estudo ecológico das estirpes Saccharomyces cerevisiae numa vinha da região dos Vinhos Verdes em Portugal. www.infowine.com, Revista Internet de Viticultura e Enologia, Nº 10/2.

O-8. Schuller, D., Alves, H., Dequin, S., Casal, M. 2006. Utilisation de souches de levures génétiquement modifiées en œnologie. www.infowine.com, Revista Internet de Viticultura e Enologia, Nº 4.

O-9. Valero, E., Schuller, D., Cambon, B., Casal, M., Dequin, S. 2006. Uso dei lieviti selezionati e biodiversità. www.infowine.com, Revista Internet de Viticultura e Enologia, Nº 3/1

O-10. Schuller, D., Alves, H., Dequin, S., Casal, M. 2006. A utilização de estirpes de leveduras geneticamente modificadas em enologia. www.infowine.com, Revista Internet de Viticultura e Enologia, Nº 4.

O-11. Schuller, D., Alves, H., Dequin, S., Casal, M. 2006. L’utilizzo di ceppi modificati di Saccharomyces cerevisiae in enologia. www.infowine.com, Revista Internet de Viticultura e Enologia, Nº 2/1.

O-12 – Membro da equipa responsável pela elaboração dos conteúdos do Centro Virtual de Desenvolvimento de Compósitos Biodegradáveis, no âmbito do projecto Naturplas e Natruplas2. http://cvnaturplas.dnsalias.com/

O-13 – Membro da equipa responsável pela elaboração dos conteúdos do Manual de Boas Práticas de Produção Vitivinícola (BPPV), no âmbito do projecto EnoSafe, 2007. A coordenação da elaboração deste Manual esteve a cargo de Margarida Casal e de António Cerdeira. Foram autores: para além dos coordenadores os seguintes elementos: Dorit Schuller (DB-UM), Sofia Machado (DB-UM), Filomenda Duarte (EVN), Ângela Campos (ACM), Fernando Moura (ACPL), Vasco Miguel (ACC) e Julião Baptista (ACC). http://www.iniap.min-agricultura.pt/ficheiros_noticias/ManualBPPV.pdf

 

Post-Docs & Students

CONCLUDED

-16 postdoc researchers, 23 PhD students, 23 Master's degree

PRESENT TEAM

Post-doc:

1. Tony Collins, investigador Ciência.

2. Raul Machado. SFRH/BPD/86470/2012/J0025076RG34

3. Isabel João Soares Silva

PhD:

1. Supervisor. Maria Eugénia Gonçalves Vieira. Bolseira de doutoramento atribuída pela ADI (SFRH/BDE/33672/2009). Starting date: 1/ June / 2010. 

2. Supervisor. David Ribas. In collaboration with Prof. Doutor George Diallinas (University of Athnes) and Prof. Sandra Paiva.   Grant SFRH/BD/96166/2013/J5874890m91z. Starting date: 1 / February / 2014.

3. Cosupervisor. Gabriel Rocha. FCT grant

4. Cosupervisor. Ana Margarida Pereira. DP-AEM student

5. Cosupervisor.Maria Silva. DP-AEM student

6. Cosupervisor. Rosana Alves. DP-AEM student

 

Latest News

You are here: Home People Members

Members' Resources